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PMID: 12513996 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Differential growth response of colony-forming alpha- and gamma-proteobacteria in dilution culture and nutrient addition experiments from Lake Kinneret (Israel), the eastern Mediterranean Sea, and the Gulf of Eilat.

Applied and environmental microbiology ·Vol. 69 ·No. 1 ·2003-01-00 ·Pages 199-211

Pinhassi J, Berman T

Abstract

Even though it is widely accepted that bacterioplankton growth in lakes and marine ecosystems is determined by the trophic status of the systems, knowledge of the relationship between nutrient concentrations and growth of particular bacterial species is almost nonexistent. To address this question, we performed a series of culture experiments with water from Lake Kinneret (Israel), the eastern Mediterranean Sea, and the Gulf of Eilat (northern Red Sea). In the initial water samples, the proportion of CFU was typically <0.002% of the 4',6'-diamidino-2-phenylindole (DAPI) counts. During incubation until the early stationary phase, the proportion of CFU increased to 20% of the DAPI counts and to 2 to 15% of the DAPI counts in unenriched lake water and seawater dilution cultures, respectively. Sequencing of the 16S ribosomal DNA of colony-forming bacteria in these cultures consistently revealed an abundance of alpha-proteobacteria, but notable phylogenetic differences were found at the genus level. Marine dilution cultures were dominated by bacteria in the Roseobacter clade, while lake dilution cultures were dominated by bacteria affiliated with the genera Sphingomonas and CAULOBACTER: In nutrient (glucose, ammonium, phosphate) addition experiments the CFU comprised 20 to 83% of the newly grown cells. In these incubation experiments fast-growing gamma-proteobacteria dominated; in the marine experiments primarily different Vibrio and Alteromonas species appeared, while in the lake water experiments species of the genera Shewanella, Aeromonas, and Rheinheimera grew. These results suggest that major, but different, gamma-proteobacterial genera in both freshwater and marine environments have a preference for elevated concentrations of nutrients and easily assimilated organic carbon sources but are selectively outcompeted by alpha-proteobacteria in the presence of low nutrient concentrations.

MeSH Terms
Alphaproteobacteria/classification,genetics,growth & development Animals Colony Count, Microbial Culture Media DNA, Ribosomal/analysis Ecosystem Fresh Water/microbiology Gammaproteobacteria/classification,genetics,growth & development Israel Molecular Sequence Data Plankton/growth & development Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Seawater/microbiology Sequence Analysis, DNA
Chemicals
Culture Media DNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pinhassi Jarone
The Yigal Allon Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, IL-14102 Tiberias, Israel. [email protected]
Berman Tom
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.69.1.199-211.2003
Email
[email protected]
Published
2003-01-00
Pages
199-211
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC152472
Subset
IM
Databases
GENBANK
AY136072, AY136073, AY136074, AY136075, AY136076, AY136077, AY136078, AY136079, AY136080, AY136081, AY136082, AY136083, AY136084, AY136085, AY136086, AY136087, AY136088, AY136089, AY136090, AY136091, AY136092, AY136093, AY136094, AY136095, AY136096, AY136097, AY136098, AY136099, AY136100, AY136101, AY136102, AY136103, AY136104, AY136105, AY136106, AY136107, AY136108, AY136109, AY136110, AY136111, AY136112, AY136113, AY136114, AY136115, AY136116, AY136117, AY136118, AY136119, AY136120, AY136121, AY136122, AY136123, AY136124, AY136125, AY136126, AY136127, AY136128, AY136129, AY136130, AY136131, AY136132, AY136133, AY136134, AY136135
External Links
PubMed source DOI: 10.1128/AEM.69.1.199-211.2003 PMC Full Text View journal details
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