Home LiteratureArticle Details
PMID: 12609877 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The lateral diffusion of selectively aggregated peptides in giant unilamellar vesicles.

Biophysical journal ·Vol. 84 ·No. 3 ·2003-03-00 ·Pages 1756-64

Lee CC, Revington M, Dunn SD, Petersen NO

Abstract

We have systematically investigated the effect of aggregation of a transmembrane peptide on its diffusion in dimyristoylphosphatidylcholine and in palmitoyloleoylphosphatidylcholine model membranes. The hydrophobic segment of the b subunit from E. coli F(1)F(0)-ATP synthase was modified with a histidine tag at the carbonyl terminus and was aggregated selectively by using a series of multivalent, dendritic chelating agents with nitrilotriacetic acid functional groups. Peptide complexes ranging from monomers to hexamers were formed and studied in giant unilamellar vesicles. The rate of diffusion for the transmembrane peptide complexes were found to depend on the size of the complex. The results agree with predictions from the free area model for monomers and dimers, and the hydrodynamic continuum model for tetramers, pentamers, and hexamers. Comparisons with diffusion of lipids confirm that the diffusion of a transmembrane peptide is enhanced by coupling of density fluctuations between the two monolayers.

MeSH Terms
Amino Acid Sequence Bacterial Proton-Translocating ATPases/chemistry,genetics,metabolism Diffusion Lipid Bilayers/chemistry Liposomes/chemistry Macromolecular Substances Membrane Fluidity Membrane Proteins/chemistry Models, Chemical Molecular Sequence Data Peptides/chemistry,genetics,metabolism Protein Subunits/chemistry Recombinant Proteins/chemistry,genetics,metabolism Sensitivity and Specificity
Chemicals
Lipid Bilayers Liposomes Macromolecular Substances Membrane Proteins Peptides Protein Subunits Recombinant Proteins Bacterial Proton-Translocating ATPases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee Clarence C
Department of Chemistry, The University of Western Ontario, London, Canada.
Revington Matthew
Dunn Stanley D
Petersen Nils O
References (50)
50 references, click to expand
  1. Cell surface organization by the membrane skeleton.
    Curr Opin Cell Biol. 1996 Aug;8(4):566-74 PMID: 8791449
  2. Membrane insertion and lateral mobility of synthetic amphiphilic signal peptides in lipid model membranes.
    Biochim Biophys Acta. 1991 Jul 22;1071(2):123-48 PMID: 1854792
  3. Effects of size of macrocyclic polyamides on their rate of diffusion in model membranes.
    Biophys J. 1997 Nov;73(5):2580-7 PMID: 9370452
  4. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels.
    J Mol Biol. 1996 Jul 19;260(3):289-98 PMID: 8757792
  5. Comment on interpretation of mechanochemical properties of lipid bilayer vesicles from the equation of state or pressure-area measurement of the monolayer at the air-water or oil-water interface.
    Langmuir. 2006 Mar 14;22(6):2916-9; discussion 2920-2 PMID: 16519504
  6. Functions of lipid rafts in biological membranes.
    Annu Rev Cell Dev Biol. 1998;14:111-36 PMID: 9891780
  7. Structure of the membrane domain of subunit b of the Escherichia coli F0F1 ATP synthase.
    J Biol Chem. 1999 May 28;274(22):15598-604 PMID: 10336456
  8. Size dependence of the translational diffusion of large integral membrane proteins in liquid-crystalline phase lipid bilayers. A study using fluorescence recovery after photobleaching.
    Biochemistry. 1982 Oct 26;21(22):5608-12 PMID: 6216914
  9. Bending elasticities of model membranes: influences of temperature and sterol content.
    Biophys J. 1997 Jun;72(6):2616-29 PMID: 9168037
  10. Metal chelate affinity chromatography, a new approach to protein fractionation.
    Nature. 1975 Dec 18;258(5536):598-9 PMID: 1678
  11. Barriers for lateral diffusion of transferrin receptor in the plasma membrane as characterized by receptor dragging by laser tweezers: fence versus tether.
    J Cell Biol. 1995 Jun;129(6):1559-74 PMID: 7790354
  12. Theory of lipid monolayer and bilayer phase transitions: effect of headgroup interactions.
    J Membr Biol. 1976;27(3):233-50 PMID: 940146
  13. Lipid rafts and B-cell activation.
    Nat Rev Immunol. 2002 Feb;2(2):96-105 PMID: 11910900
  14. The red-edge effects: 30 years of exploration.
    Luminescence. 2002 Jan-Feb;17(1):19-42 PMID: 11816059
  15. Extraction of membrane microviscosity from translational and rotational diffusion coefficients.
    Biophys J. 1982 Mar;37(3):673-6 PMID: 7074193
  16. Regulation of band 3 mobilities in erythrocyte ghost membranes by protein association and cytoskeletal meshwork.
    Biochemistry. 1988 Sep 20;27(19):7447-52 PMID: 2462903
  17. Submicron structure in L-alpha-dipalmitoylphosphatidylcholine monolayers and bilayers probed with confocal, atomic force, and near-field microscopy.
    Biophys J. 1998 Jul;75(1):342-53 PMID: 9649391
  18. AKAPs: from structure to function.
    Trends Cell Biol. 1999 Jun;9(6):216-21 PMID: 10354567
  19. Measurement of membrane protein lateral diffusion in single cells.
    Science. 1976 Feb 6;191(4226):466-8 PMID: 1246629
  20. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.
    Science. 2002 May 3;296(5569):913-6 PMID: 11988576
  21. Translational diffusion of lipids in liquid crystalline phase phosphatidylcholine multibilayers. A comparison of experiment with theory.
    Biochemistry. 1985 Jan 29;24(3):781-6 PMID: 3994985
  22. Lateral and rotational diffusion of bacteriorhodopsin in lipid bilayers: experimental test of the Saffman-Delbrück equations.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4317-21 PMID: 6956861
  23. Periodic structures in lipid monolayer phase transitions.
    Proc Natl Acad Sci U S A. 1984 May;81(10):3249-53 PMID: 16593467
  24. A comparison of the translational diffusion of a normal and a membrane-spanning lipid in L alpha phase 1-palmitoyl-2-oleoylphosphatidylcholine bilayers.
    Eur Biophys J. 1985;12(1):19-24 PMID: 3924584
  25. A model for the lateral diffusion of "stiff" chains in a lipid bilayer.
    Eur Biophys J. 1985;12(1):25-31 PMID: 4006877
  26. PDZ domains in synapse assembly and signalling.
    Trends Cell Biol. 2000 Jul;10(7):274-80 PMID: 10856930
  27. Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells.
    Biophys J. 1993 Nov;65(5):2021-40 PMID: 8298032
  28. Comparative lateral diffusion of fluorescent lipid analogues in phospholipid multibilayers.
    Biochemistry. 1980 Dec 23;19(26):6050-7 PMID: 7470449
  29. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
    Biophys J. 1976 Sep;16(9):1055-69 PMID: 786399
  30. Structure of lipid bilayers.
    Biochim Biophys Acta. 2000 Nov 10;1469(3):159-95 PMID: 11063882
  31. More on the motional state of lipid bilayer membranes: interpretation of order parameters obtained from nuclear magnetic resonance experiments.
    Biochemistry. 1977 Jun 14;16(12):2657-67 PMID: 889782
  32. Lateral diffusion in model membranes is independent of the size of the hydrophobic region of molecules.
    Biophys J. 1993 Aug;65(2):630-7 PMID: 8218892
  33. The size of lipid rafts: an atomic force microscopy study of ganglioside GM1 domains in sphingomyelin/DOPC/cholesterol membranes.
    Biophys J. 2002 May;82(5):2526-35 PMID: 11964241
  34. Lateral mobility of erythrocyte membrane proteins studied by the fluorescence photobleaching recovery technique.
    J Biochem. 1981 Oct;90(4):997-1004 PMID: 6458603
  35. Structure and origin of ordered lipid domains in biological membranes.
    J Membr Biol. 1998 Jul 15;164(2):103-14 PMID: 9662555
  36. Compartmentalized structure of the plasma membrane for receptor movements as revealed by a nanometer-level motion analysis.
    J Cell Biol. 1994 Jun;125(6):1251-64 PMID: 8207056
  37. Roles of lipid rafts in membrane transport.
    Curr Opin Cell Biol. 2001 Aug;13(4):470-7 PMID: 11454454
  38. Two-photon fluorescence microscopy observation of shape changes at the phase transition in phospholipid giant unilamellar vesicles.
    Biophys J. 1999 Oct;77(4):2090-101 PMID: 10512829
  39. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  40. The dynamic structure of fatty acyl chains in a phospholipid bilayer measured by deuterium magnetic resonance.
    Biochemistry. 1974 Nov 5;13(23):4839-45 PMID: 4371820
  41. Lipid rafts and signal transduction.
    Nat Rev Mol Cell Biol. 2000 Oct;1(1):31-9 PMID: 11413487
  42. On two-dimensional passive random walk in lipid bilayers and fluid pathways in biomembranes.
    J Membr Biol. 1979 Jul 31;48(3):215-36 PMID: 40032
  43. Effect of the structure of natural sterols and sphingolipids on the formation of ordered sphingolipid/sterol domains (rafts). Comparison of cholesterol to plant, fungal, and disease-associated sterols and comparison of sphingomyelin, cerebrosides, and ceramide.
    J Biol Chem. 2001 Sep 7;276(36):33540-6 PMID: 11432870
  44. Weak dependence of mobility of membrane protein aggregates on aggregate size supports a viscous model of retardation of diffusion.
    Biophys J. 1999 Jan;76(1 Pt 1):314-22 PMID: 9876143
  45. Fibronectin receptor exhibits high lateral mobility in embryonic locomoting cells but is immobile in focal contacts and fibrillar streaks in stationary cells.
    J Cell Biol. 1988 Oct;107(4):1385-96 PMID: 2971668
  46. Restriction of the lateral motion of band 3 in the erythrocyte membrane by the cytoskeletal network: dependence on spectrin association state.
    Biochemistry. 1986 Oct 7;25(20):6133-9 PMID: 3790510
  47. Brownian motion in biological membranes.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):3111-3 PMID: 1059096
  48. Translational diffusion in phospholipid monolayers measured by fluorescence microphotolysis.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7183-7 PMID: 6580635
  49. The differential miscibility of lipids as the basis for the formation of functional membrane rafts.
    Biochim Biophys Acta. 1998 Nov 10;1376(3):467-79 PMID: 9805010
  50. Translational diffusion of acetylcholine receptor (monomeric and dimeric forms) of Torpedo marmorata reconstituted into phospholipid bilayers studied by fluorescence recovery after photobleaching.
    Biochemistry. 1982 Nov 9;21(23):5750-5 PMID: 6897514
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/s0006-3495(03)74983-2
Published
2003-03-00
Pages
1756-64
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1302744
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1016/s0006-3495(03)74983-2 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2