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PMID: 2971668 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Fibronectin receptor exhibits high lateral mobility in embryonic locomoting cells but is immobile in focal contacts and fibrillar streaks in stationary cells.

The Journal of cell biology ·Vol. 107 ·No. 4 ·1988-10-00 ·Pages 1385-96

Duband JL, Nuckolls GH, Ishihara A, Hasegawa T, Yamada KM, Thiery JP, Jacobson K

Abstract

The dynamic process of embryonic cell motility was investigated by analyzing the lateral mobility of the fibronectin receptor in various locomotory or stationary avian embryonic cells, using the technique of fluorescence recovery after photobleaching. The lateral mobility of fibronectin receptors, labeled by a monoclonal antibody, was defined by the diffusion coefficient and mobile fraction of these receptors. Even though the lateral diffusion coefficient did not vary appreciably (2 X 10(-10) cm2/S less than or equal to D less than or equal to 4 X 10(-10) cm2/S) with the locomotory state and the cell type, the mobile fraction was highly dependent on the degree of cell motility. In locomoting cells, the population of fibronectin receptors, which was uniformly distributed on the cell surface, displayed a high mobile fraction of 66 +/- 19% at 25 degrees C (82 +/- 14% at 37 degrees C). In contrast, in nonmotile cells, the population of receptors was concentrated in focal contacts and fibrillar streaks associated with microfilament bundles and, in these sites, the mobile fraction was small (16 +/- 8%). When cells were in a stage intermediate between highly motile and stationary, the population of fibronectin receptors was distributed both in focal contacts with a small mobile fraction and in a diffuse pattern with a reduced mobile fraction (33 +/- 9%) relative to the diffuse population in highly locomotory cells. The mobile fraction of the fibronectin receptor was found to be temperature dependent in locomoting but not in stationary cells. The mobile fraction could be modulated by affecting the interaction between the receptor and the substratum. The strength of this interaction could be increased by growing cells on a substratum coated with polyclonal antibodies to the receptor. This caused the mobile fraction to decrease. The interaction could be decreased by using a probe, monoclonal antibodies to the receptor known to perturb the adhesion of certain cell types which caused the mobile fraction to increase. From these results, we conclude that in locomoting embryonic cells, most fibronectin receptors can readily diffuse in the plane of the membrane. This degree of lateral mobility may be correlated to the labile adhesions to the substratum presumably required for high motility. In contrast, fibronectin receptors in stationary cells are immobilized in focal contacts and fibrillar streaks which are in close association with both extracellular and cytoskeletal structures; these stable complexes appear to provide firm anchorage to the substratum.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Cell Adhesion Cell Movement Chick Embryo Coturnix Fibronectins/physiology Fluorescent Antibody Technique Membrane Fluidity Neural Crest/cytology Receptors, Fibronectin Receptors, Immunologic/physiology Temperature
Chemicals
Antibodies, Monoclonal Fibronectins Receptors, Fibronectin Receptors, Immunologic
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Duband J L
Centre National de la Recherche Scientifique, Ecole Normale Supérieure, Paris, France.
Nuckolls G H
Ishihara A
Hasegawa T
Yamada K M
Thiery J P
Jacobson K
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.4.1385
Published
1988-10-00
Pages
1385-96
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115255
Subset
IM
Grants
NIGMS NIH HHS · GM 29234 · United States
External Links
PubMed source DOI: 10.1083/jcb.107.4.1385 PMC Full Text View journal details
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