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PMID: 12612077 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Intersection of the Kap123p-mediated nuclear import and ribosome export pathways.

Molecular and cellular biology ·Vol. 23 ·No. 6 ·2003-03-00 ·Pages 2042-54

Sydorskyy Y, Dilworth DJ, Yi EC, Goodlett DR, Wozniak RW, Aitchison JD

Abstract

Kap123p is a yeast beta-karyopherin that imports ribosomal proteins into the nucleus prior to their assembly into preribosomal particles. Surprisingly, Kap123p is not essential for growth, under normal conditions. To further explore the role of Kap123p in nucleocytoplasmic transport and ribosome biogenesis, we performed a synthetic fitness screen designed to identify genes that interact with KAP123. Through this analysis we have identified three other karyopherins, Pse1p/Kap121p, Sxm1p/Kap108p, and Nmd5p/Kap119p. We propose that, in the absence of Kap123p, these karyopherins are able to supplant Kap123p's role in import. In addition to the karyopherins, we identified Rai1p, a protein previously implicated in rRNA processing. Rai1p is also not essential, but deletion of the RAI1 gene is deleterious to cell growth and causes defects in rRNA processing, which leads to an imbalance of the 60S/40S ratio and the accumulation of halfmers, 40S subunits assembled on polysomes that are unable to form functional ribosomes. Rai1p localizes predominantly to the nucleus, where it physically interacts with Rat1p and pre-60S ribosomal subunits. Analysis of the rai1/kap123 double mutant strain suggests that the observed genetic interaction results from an inability to efficiently export pre-60S subunits from the nucleus, which arises from a combination of compromised Kap123p-mediated nuclear import of the essential 60S ribosomal subunit export factor, Nmd3p, and a DeltaRAI1-induced decrease in the overall biogenesis efficiency.

MeSH Terms
Active Transport, Cell Nucleus/physiology Alleles Carrier Proteins/genetics,physiology Cell Nucleus/metabolism Exoribonucleases/metabolism Karyopherins/physiology Membrane Transport Proteins Nuclear Proteins/genetics,physiology RNA Precursors/metabolism RNA, Fungal/metabolism RNA-Binding Proteins/physiology Receptors, Cytoplasmic and Nuclear/physiology Ribosomes/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism,physiology beta Karyopherins
Chemicals
Carrier Proteins Kap123 protein, S cerevisiae Karyopherins Membrane Transport Proteins NMD3 protein, S cerevisiae NMD5 protein, S cerevisiae Nuclear Proteins PSE1 protein, S cerevisiae RNA Precursors RNA, Fungal RNA-Binding Proteins Rai1 protein, S cerevisiae Receptors, Cytoplasmic and Nuclear SXM1 protein, S cerevisiae Saccharomyces cerevisiae Proteins beta Karyopherins RAT1 protein, S cerevisiae Exoribonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sydorskyy Y
Institute for Systems Biology, 1441 N 34th Street, Seattle, Washington 98105, USA.
Dilworth D J
Yi E C
Goodlett D R
Wozniak R W
Aitchison J D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.6.2042-2054.2003
Published
2003-03-00
Pages
2042-54
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC149464
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.23.6.2042-2054.2003 PMC Full Text View journal details
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