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PMID: 9238021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Importin/karyopherin protein family members required for mRNA export from the nucleus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 16 ·1997-08-05 ·Pages 8590-5

Seedorf M, Silver PA

Abstract

The yeast Saccharomyces cerevisiae contains three proteins (Kap104p, Pse1p, and Kap123p) that share similarity to the 95-kDa beta subunit of the nuclear transport factor importin (also termed karyopherin and encoded by KAP95/RSL1 in yeast). Proteins that contain nuclear localization sequences are recognized in the cytoplasm and delivered to the nucleus by the heterodimeric importin complex. A second importin-related protein, transportin, delivers a subset of heterogeneous nuclear ribonucleoproteins (hnRNPs) to the nucleoplasm. We now show that in contrast to loss of importin beta (Kap95p/Rsl1p) and transportin (Kap104p), conditional loss of Pse1p in a strain lacking Kap123p results in a specific block of mRNA export from the nucleus. Overexpression of Sxm1p, a protein related to Cse1p in yeast and to the human cellular apoptosis susceptibility protein, relieves the defects of cells lacking Pse1p and Kap123p. Thus, a major role of Pse1p, Kap123p, and Sxm1p may be nuclear export rather than import, suggesting a symmetrical relationship between these processes.

MeSH Terms
Biological Transport Cell Nucleus/metabolism Fungal Proteins/genetics,metabolism Humans Karyopherins Mutation Nuclear Proteins/genetics,metabolism RNA, Fungal/metabolism RNA, Messenger/metabolism Saccharomyces cerevisiae/metabolism
Chemicals
Fungal Proteins Karyopherins Nuclear Proteins RNA, Fungal RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Seedorf M
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
Silver P A
References (50)
50 references, click to expand
  1. Potential RNA binding proteins in Saccharomyces cerevisiae identified as suppressors of temperature-sensitive mutations in NPL3.
    Genetics. 1996 Jan;142(1):103-15 PMID: 8770588
  2. The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo.
    J Biol Chem. 1996 Aug 2;271(31):18477-84 PMID: 8702493
  3. Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins.
    Science. 1996 Oct 25;274(5287):624-7 PMID: 8849456
  4. A GTPase controlling nuclear trafficking: running the right way or walking RANdomly?
    Cell. 1996 Oct 4;87(1):1-4 PMID: 8858141
  5. Importin provides a link between nuclear protein import and U snRNA export.
    Cell. 1996 Oct 4;87(1):21-32 PMID: 8858145
  6. In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation.
    J Cell Biol. 1996 Oct;135(2):329-39 PMID: 8896592
  7. Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae.
    Science. 1996 Nov 15;274(5290):1201-4 PMID: 8895471
  8. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
    EMBO J. 1997 Feb 17;16(4):807-16 PMID: 9049309
  9. Yeast recombination: the association between double-strand gap repair and crossing-over.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4417-21 PMID: 6308623
  10. Amino acid sequences that determine the nuclear localization of yeast histone 2B.
    Mol Cell Biol. 1987 Nov;7(11):4048-57 PMID: 3123916
  11. Context affects nuclear protein localization in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Feb;9(2):384-9 PMID: 2496300
  12. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  13. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  14. A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.
    EMBO J. 1989 Dec 20;8(13):4015-24 PMID: 2686980
  15. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers.
    Yeast. 1990 Sep-Oct;6(5):363-6 PMID: 2220072
  16. Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import.
    Cell. 1991 Sep 6;66(5):837-47 PMID: 1653647
  17. Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.
    Genes Dev. 1992 Jul;6(7):1173-89 PMID: 1628825
  18. Screening and identification of a gene, PSE-1, that affects protein secretion in Saccharomyces cerevisiae.
    J Cell Sci. 1992 Mar;101 ( Pt 3):709-19 PMID: 1522152
  19. A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast.
    Mol Biol Cell. 1992 Aug;3(8):875-93 PMID: 1392078
  20. Cloning and characterization of SRP1, a suppressor of temperature-sensitive RNA polymerase I mutations, in Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Dec;12(12):5640-51 PMID: 1448093
  21. NAB2: a yeast nuclear polyadenylated RNA-binding protein essential for cell viability.
    Mol Cell Biol. 1993 May;13(5):2730-41 PMID: 8474438
  22. Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily.
    EMBO J. 1993 Jul;12(7):2929-37 PMID: 7687541
  23. CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae.
    Mol Cell Biol. 1993 Aug;13(8):4691-702 PMID: 8336709
  24. The GTP-binding protein Ran/TC4 is required for protein import into the nucleus.
    Nature. 1993 Oct 14;365(6447):661-3 PMID: 8413630
  25. Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae.
    J Cell Biol. 1994 Dec;127(5):1173-84 PMID: 7962083
  26. A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm.
    Mol Cell Biol. 1994 Dec;14(12):8399-407 PMID: 7969175
  27. Isolation of a protein that is essential for the first step of nuclear protein import.
    Cell. 1994 Dec 2;79(5):767-78 PMID: 8001116
  28. The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm.
    Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):225-9 PMID: 7816822
  29. Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins.
    Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1769-73 PMID: 7878057
  30. Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.
    Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2008-11 PMID: 7892216
  31. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C.
    Yeast. 1995 Jan;11(1):53-5 PMID: 7762301
  32. Sequence and characterization of cytoplasmic nuclear protein import factor p97.
    J Cell Biol. 1995 Jul;130(2):265-74 PMID: 7615630
  33. Nuclear export signals and the fast track to the cytoplasm.
    Cell. 1995 Aug 11;82(3):341-4 PMID: 7634321
  34. In vivo evidence for involvement of a 58 kDa component of nuclear pore-targeting complex in nuclear protein import.
    EMBO J. 1995 Aug 1;14(15):3617-26 PMID: 7641681
  35. The yeast nuclear import receptor is required for mitosis.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7647-51 PMID: 7644471
  36. A cap-binding protein complex mediating U snRNA export.
    Nature. 1995 Aug 24;376(6542):709-12 PMID: 7651522
  37. Rna1p, a Ran/TC4 GTPase activating protein, is required for nuclear import.
    J Cell Biol. 1995 Sep;130(5):1017-26 PMID: 7657689
  38. Distinct functions for the two importin subunits in nuclear protein import.
    Nature. 1995 Sep 21;377(6546):246-8 PMID: 7675110
  39. Kinetics of spindle pole body separation in budding yeast.
    Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9707-11 PMID: 7568202
  40. Yeast Srp1, a nuclear protein related to Drosophila and mouse pendulin, is required for normal migration, division, and integrity of nuclei during mitosis.
    Mol Gen Genet. 1995 Aug 21;248(3):351-63 PMID: 7565597
  41. Cloning and characterization of a cellular apoptosis susceptibility gene, the human homologue to the yeast chromosome segregation gene CSE1.
    Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10427-31 PMID: 7479798
  42. GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import.
    J Cell Biol. 1995 Nov;131(3):571-81 PMID: 7593180
  43. A pre-mRNA-binding protein accompanies the RNA from the gene through the nuclear pores and into polysomes.
    Cell. 1996 Jan 26;84(2):253-64 PMID: 8565071
  44. Nucleocytoplasmic transport.
    Science. 1996 Mar 15;271(5255):1513-8 PMID: 8599106
  45. A nuclear cap-binding complex binds Balbiani ring pre-mRNA cotranscriptionally and accompanies the ribonucleoprotein particle during nuclear export.
    J Cell Biol. 1996 Apr;133(1):5-14 PMID: 8601613
  46. A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export.
    Genes Dev. 1996 May 15;10(10):1233-46 PMID: 8675010
  47. Role of CAS, a human homologue to the yeast chromosome segregation gene CSE1, in toxin and tumor necrosis factor mediated apoptosis.
    Biochemistry. 1996 May 28;35(21):6891-9 PMID: 8639641
  48. Regulation of mRNA export in response to stress in Saccharomyces cerevisiae.
    Genes Dev. 1996 Jul 1;10(13):1608-20 PMID: 8682292
  49. Dynamic localization of the nuclear import receptor and its interactions with transport factors.
    J Cell Biol. 1996 Jun;133(6):1163-76 PMID: 8682856
  50. A novel receptor-mediated nuclear protein import pathway.
    Cell. 1996 Sep 20;86(6):985-94 PMID: 8808633
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.16.8590
Published
1997-08-05
Pages
8590-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23031
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.94.16.8590 PMC Full Text View journal details
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