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PMID: 7816822 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 1 ·1995-01-03 ·Pages 225-9

Schlenstedt G, Saavedra C, Loeb JD, Cole CN, Silver PA

Abstract

Ran/TC4, a Ras-like GTP-binding protein, and its nucleotide exchanger, RCC1, have been implicated in control of protein movement into the nucleus and cytoplasmic accumulation of mRNA. Saccharomyces cerevisiae contains two homologues of the mammalian Ran/TC4, encoded by the GSP1 and GSP2 genes. We have constructed yeast strains that overproduce either wild-type Gsp1 or a form of Gsp1 with glycine-21 converted to valine (Gsp1-G21V), which we show stabilizes the GTP-bound form. Cells producing Gsp1-G21V have defects in localization of nuclear proteins; nuclear proteins accumulate in the cytoplasm following galactose induction of Gsp1-G21V. Similarly, cells producing Gsp1-G21V retain poly(A)+ RNA in their nuclei. These findings suggest that hydrolysis of GTP by Ran/TC4 is necessary for proper import of proteins into the nucleus and appearance of poly(A)+ RNA in the cytoplasm.

Related Genes
GSP1 GSP2
MeSH Terms
Animals Cell Nucleus/metabolism Cytoplasm/metabolism GTP Phosphohydrolases/metabolism GTP-Binding Proteins/genetics,metabolism Genes, Fungal Guanosine Triphosphate/metabolism Kinetics Mammals Microscopy, Fluorescence Nuclear Proteins/biosynthesis,genetics,metabolism RNA, Messenger/metabolism Recombinant Fusion Proteins/biosynthesis Saccharomyces cerevisiae/genetics,growth & development,metabolism ran GTP-Binding Protein
Chemicals
Nuclear Proteins RNA, Messenger Recombinant Fusion Proteins Guanosine Triphosphate GTP Phosphohydrolases GTP-Binding Proteins ran GTP-Binding Protein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schlenstedt G
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA.
Saavedra C
Loeb J D
Cole C N
Silver P A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.1.225
Published
1995-01-03
Pages
225-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42850
Subset
IM
Grants
NIGMS NIH HHS · R01 GM033998 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.1.225 PMC Full Text View journal details
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