Home LiteratureArticle Details
PMID: 7969175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm.

Molecular and cellular biology ·Vol. 14 ·No. 12 ·1994-12-00 ·Pages 8399-407

Flach J, Bossie M, Vogel J, Corbett A, Jinks T, Willins DA, Silver PA

Abstract

RNA-binding proteins have been suggested to move in association with RNA as it leaves the nucleus. The NPL3 gene of the yeast Saccharomyces cerevisiae encodes in nuclear protein with consensus RNA-binding motifs and similarity to heterogeneous nuclear ribonucleoproteins and members of the S/R protein family. We show that although Npl3 is located in the nucleus, it can shuttle between nuclei in yeast heterokaryons. In contrast, other nucleus-targeted proteins do not leave the nucleus under similar conditions. Mutants missing the RNA-binding motifs or the N terminus are still capable of shuttling in and out of the nucleus. Npl3 mutants missing the C terminus fail to localize to the nucleus. Overproduction of Npl3 in wild-type cells shows cell growth. This toxicity depends on the presence of series of unique repeats in the N terminus and localization to the nucleus. We suggest that the properties of Npl3 are consistent with it being involved in export of RNAs from the nucleus.

Related Genes
NPL3
MeSH Terms
Biological Transport Cell Compartmentation Cell Nucleus/metabolism Cytoplasm/metabolism DNA Mutational Analysis Fungal Proteins/metabolism Nuclear Proteins/metabolism RNA-Binding Proteins/metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Structure-Activity Relationship
Chemicals
Fungal Proteins NPL3 protein, S cerevisiae Nuclear Proteins RNA-Binding Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Flach J
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
Bossie M
Vogel J
Corbett A
Jinks T
Willins D A
Silver P A
References (33)
33 references, click to expand
  1. Identification of two HSP70-related Xenopus oocyte proteins that are capable of recycling across the nuclear envelope.
    J Cell Biol. 1990 Nov;111(5 Pt 1):1775-83 PMID: 2229173
  2. Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen.
    J Biol Chem. 1990 Feb 5;265(4):2209-15 PMID: 2298745
  3. Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
    Nature. 1992 Feb 20;355(6362):730-2 PMID: 1371331
  4. Export of mRNA from microinjected nuclei of Xenopus laevis oocytes.
    J Cell Biol. 1992 Jul;118(1):1-9 PMID: 1618896
  5. Nopp140 shuttles on tracks between nucleolus and cytoplasm.
    Cell. 1992 Jul 10;70(1):127-38 PMID: 1623516
  6. A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast.
    Mol Biol Cell. 1992 Aug;3(8):875-93 PMID: 1392078
  7. NOP3 is an essential yeast protein which is required for pre-rRNA processing.
    J Cell Biol. 1992 Nov;119(4):737-47 PMID: 1429834
  8. Nuclear export of proteins: the role of nuclear retention.
    Cell. 1993 Aug 13;74(3):493-504 PMID: 8348616
  9. hnRNP proteins and the biogenesis of mRNA.
    Annu Rev Biochem. 1993;62:289-321 PMID: 8352591
  10. Nuclear shuttling: the default pathway for nuclear proteins?
    Cell. 1993 Aug 27;74(4):585-6 PMID: 8358787
  11. Reconstitution of nuclear protein transport with semi-intact yeast cells.
    J Cell Biol. 1993 Nov;123(4):785-98 PMID: 8227140
  12. Analysis of nucleo-cytoplasmic transport in a thermosensitive mutant of nuclear pore protein NSP1.
    Eur J Cell Biol. 1993 Oct;62(1):1-12 PMID: 8269967
  13. Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors.
    Nucleic Acids Res. 1993 Dec 25;21(25):5803-16 PMID: 8290338
  14. Green fluorescent protein as a marker for gene expression.
    Science. 1994 Feb 11;263(5148):802-5 PMID: 8303295
  15. The karyogamy gene KAR2 and novel proteins are required for ER-membrane fusion.
    Cell. 1994 Jul 15;78(1):87-98 PMID: 8033215
  16. A mutant of Saccharomyces cerevisiae defective for nuclear fusion.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3651-5 PMID: 790391
  17. A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
    Cell. 1982 Sep;30(2):449-58 PMID: 6814762
  18. Visualization of the formation and transport of a specific hnRNP particle.
    Cell. 1983 Oct;34(3):847-55 PMID: 6556087
  19. tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
    Proc Natl Acad Sci U S A. 1983 Nov;80(21):6436-40 PMID: 6579529
  20. Targeting of E. coli beta-galactosidase to the nucleus in yeast.
    Cell. 1984 Apr;36(4):1057-65 PMID: 6323016
  21. Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5951-5 PMID: 6091123
  22. Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Oct;4(10):1985-98 PMID: 6390181
  23. A short amino acid sequence able to specify nuclear location.
    Cell. 1984 Dec;39(3 Pt 2):499-509 PMID: 6096007
  24. Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
    J Cell Biol. 1987 Feb;104(2):189-200 PMID: 3805121
  25. Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
    Cell. 1988 Mar 11;52(5):641-53 PMID: 3345567
  26. Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.
    Cell. 1988 Mar 11;52(5):655-64 PMID: 3125984
  27. Translocation of RNA-coated gold particles through the nuclear pores of oocytes.
    J Cell Biol. 1988 Mar;106(3):575-84 PMID: 2450095
  28. A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.
    J Cell Biol. 1988 Oct;107(4):1289-97 PMID: 2459127
  29. Nuclear transport of adenovirus DNA polymerase is facilitated by interaction with preterminal protein.
    Cell. 1988 Dec 23;55(6):1005-15 PMID: 3203379
  30. Major nucleolar proteins shuttle between nucleus and cytoplasm.
    Cell. 1989 Feb 10;56(3):379-90 PMID: 2914325
  31. Context affects nuclear protein localization in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Feb;9(2):384-9 PMID: 2496300
  32. A hitchhiker's guide to analysis of the secretory pathway in yeast.
    Methods Cell Biol. 1989;32:3-36 PMID: 2691854
  33. A nuclear localization signal binding protein in the nucleolus.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2235-45 PMID: 2177472
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.12.8399-8407.1994
Published
1994-12-00
Pages
8399-407
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359379
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.12.8399-8407.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2