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PMID: 8290338 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors.

Nucleic acids research ·Vol. 21 ·No. 25 ·1993-12-25 ·Pages 5803-16

Birney E, Kumar S, Krainer AR

Abstract

We present a systematic analysis of sequence motifs found in metazoan protein factors involved in constitutive pre-mRNA splicing and in alternative splicing regulation. Using profile analysis we constructed a database enriched in protein sequences containing one or more presumptive copies of the RNA-recognition motif (RRM). We provide an accurate alignment of RRMs and structure-based criteria for identifying new RRMs, including many that lack the prototype RNP-1 submotif. We present a comprehensive table of 125 sequences containing 252 RRMs, including 22 previously unreported RRMs in 17 proteins. The presence of a putative RRM in these proteins, which are implicated in a variety of cellular processes, strongly suggests that their function involves binding to RNA. Unreported homologies in the RRM-enriched database to the metazoan SR family of splicing factors are described for an Arg-rich human nuclear protein and two yeast proteins (S. pombe mei2 and S. cerevisiae Npl3). We have rigorously tested the phylogenetic relationships of a large sample of RRMs. This analysis indicates that the RRM is an ancient conserved region (ACR) that has diversified by duplication of genes and intragenic domains. Statistical analyses and classification of repeated Arg-Ser (RS) and RGG domains in various protein splicing factors are presented.

MeSH Terms
Amino Acid Sequence Animals Arginine/metabolism Binding Sites Conserved Sequence Glycine/metabolism Humans Information Systems Molecular Sequence Data Phylogeny RNA Precursors/metabolism RNA Splicing RNA-Binding Proteins/chemistry,classification,metabolism Sequence Homology, Amino Acid Serine/metabolism
Chemicals
RNA Precursors RNA-Binding Proteins Serine Arginine Glycine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Birney E
Cold Spring Harbor Laboratory, NY 11724-2208.
Kumar S
Krainer A R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.25.5803
Published
1993-12-25
Pages
5803-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310458
Subset
IM
Grants
NIGMS NIH HHS · GM15262 · United States
NIGMS NIH HHS · GM42699 · United States
External Links
PubMed source DOI: 10.1093/nar/21.25.5803 PMC Full Text View journal details
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