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PMID: 1714384 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of a yeast U1 snRNP 70K protein homologue: functional conservation of an RNA-binding domain between humans and yeast.

The EMBO journal ·Vol. 10 ·No. 9 ·1991-09-00 ·Pages 2627-34

Smith V, Barrell BG

Abstract

We have cloned and sequenced a gene encoding a yeast homologue of the U1 snRNP 70K protein. The gene, SNP1, encodes a protein which has 30% amino acid identity with the human 70K protein and has a predicted molecular weight of 34 kDa. The yeast and human sequences are more closely related to each other than to other (non-U1) RNA-binding proteins, but diverge considerably in their C-terminal portions. In particular, SNP1 lacks the charged carboxy terminus of the human 70K protein. A yeast strain, a alpha 115, was constructed in which one allele of the SNP1 gene contained a 554 bp deletion. Tetrad analysis of a alpha 115 showed that the SNP1 gene is essential for the viability of yeast cells. The complete human 70K gene did not complement snp1, but the lethal snp1 mutation was rescued by plasmids bearing a chimera in which over half the yeast gene was replaced with the homologous region of the human 70K gene, including the RNA-binding domain. These results suggest that SNP1 encodes a functional homologue of the U1 snRNP 70K protein.

Related Genes
SNP1
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular DNA Probes DNA, Fungal/genetics Genes, Fungal Genes, Lethal Humans Molecular Sequence Data Mutation Polymerase Chain Reaction RNA/metabolism RNA, Fungal/metabolism Restriction Mapping Ribonucleoproteins/genetics Ribonucleoproteins, Small Nuclear Saccharomyces cerevisiae/genetics Sequence Alignment
Chemicals
DNA Probes DNA, Fungal RNA, Fungal Ribonucleoproteins Ribonucleoproteins, Small Nuclear RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith V
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Barrell B G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-09-00
Pages
2627-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452962
Subset
IM
Databases
GENBANK
X59986
External Links
PubMed source PMC Full Text View journal details
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