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PMID: 3323885 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast pre-mRNA splicing requires a minimum distance between the 5' splice site and the internal branch acceptor site.

Molecular and cellular biology ·Vol. 7 ·No. 11 ·1987-11-00 ·Pages 4010-6

Thompson-Jäger S, Domdey H

Abstract

We have generated several deletions within the intron of a yeast actin gene construct which have lead to different splicing efficiencies as measured by Northern blot (RNA blot) and primer extension analyses. Our data especially demonstrate that a minimum distance from the 5' splice site to the internal branch acceptor site is required for accurate and efficient splicing. In a construct in which splicing was completely abolished, splicing could be restored by expanding the distance from the 5' splice site to the internal branch acceptor site with heterologous sequences. Alternative splicing, i.e., exon skipping and the use of a cryptic 5' splice site, was observed when the mRNA precursor was derived from a tandem repeat of a truncated intron with flanking exon sequences.

MeSH Terms
Actins/genetics Base Sequence Chromosome Deletion Escherichia coli/genetics Genes Genes, Fungal Introns Mutation RNA Precursors/genetics RNA Splicing Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Actins RNA Precursors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thompson-Jäger S
Laboratorium für Molekulare Biologie, Ludwig-Maximilians-Universität München, Martinsried, Federal Republic of Germany.
Domdey H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.11.4010-4016.1987
Published
1987-11-00
Pages
4010-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368070
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.7.11.4010-4016.1987 PMC Full Text View journal details
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