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PMID: 3857622 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

mRNA precursor splicing in vivo: sequence requirements determined by deletion analysis of an intervening sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 9 ·1985-05-00 ·Pages 2885-9

van Santen VL, Spritz RA

Abstract

To define the extent of intervening sequence required for splicing higher eukaryotic mRNA precursors in vivo, we constructed deletions within the second intervening sequence of the human G gamma-globin gene that progressively approach the donor or acceptor splice sites. Most of the intervening sequence can be deleted with no effect on splicing. At the donor splice site, 6 bases of intervening sequence are sufficient for accurate and efficient splicing. At the acceptor splice site, 20 bases are sufficient for accurate and efficient splicing, and 16 bases are sufficient for accurate splicing but at a reduced level. However, 15 bases are insufficient for splicing at a significant level. The effect of deletions ending near the acceptor splice site is independent of whether an A-G dinucleotide is introduced into the acceptor splice site region by the deletion.

MeSH Terms
Base Sequence Chromosome Deletion Globins/genetics Humans Nucleic Acid Precursors/genetics Plasmids RNA Splicing RNA, Messenger/genetics Transfection
Chemicals
Nucleic Acid Precursors RNA, Messenger Globins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
van Santen V L
Spritz R A
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.9.2885
Published
1985-05-00
Pages
2885-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397671
Subset
IM
Grants
NIADDK NIH HHS · AM28598 · United States
NIGMS NIH HHS · GM07131 · United States
Databases
GENBANK
J00093, J00094, J00096, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179, K01239, K01890, K02544, M18047, M19067, X00423
External Links
PubMed source DOI: 10.1073/pnas.82.9.2885 PMC Full Text View journal details
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