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PMID: 3023896 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Activation of a cryptic TACTAAC box in the Saccharomyces cerevisiae actin intron.

Molecular and cellular biology ·Vol. 6 ·No. 5 ·1986-05-00 ·Pages 1571-8

Cellini A, Parker R, McMahon J, Guthrie C, Rossi J

Abstract

We constructed a translational fusion between the Saccharomyces cerevisiae actin gene and the Escherichia coli beta-galactosidase structural gene such that expression of beta-galactosidase activity required accurate splicing of the actin intron. Using this chimeric gene, we generated a series of internal deletions which removed the TACTAAC box or, in addition, TACTAAC-like sequences within the intron. Analysis of the fusion transcripts produced in these deletions allowed us to conclude that the TACTAAC-like sequence TACTAAG can substitute, albeit inefficiently, for the authentic TACTAAC box in the splicing process. These results indicate that the yeast splicing machinery can utilize a cryptic TACTAAC box, but there are requirements for primary sequence and proper position.

MeSH Terms
Actins/genetics Base Sequence Chromosome Deletion DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Genes, Fungal Plasmids Protein Biosynthesis RNA Splicing Saccharomyces cerevisiae/genetics beta-Galactosidase/genetics
Chemicals
Actins DNA Restriction Enzymes beta-Galactosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cellini A
Parker R
McMahon J
Guthrie C
Rossi J
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33 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.5.1571-1578.1986
Published
1986-05-00
Pages
1571-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367683
Subset
IM
Grants
NIGMS NIH HHS · 5T32 GM07810-05 · United States
NIGMS NIH HHS · GM21119 · United States
NIGMS NIH HHS · GM30134 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.5.1571-1578.1986 PMC Full Text View journal details
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