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PMID: 6091033 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural comparison of yeast ribosomal protein genes.

Nucleic acids research ·Vol. 12 ·No. 17 ·1984-09-11 ·Pages 6685-700

Leer RJ, van Raamsdonk-Duin MM, Hagendoorn MJ, Mager WH, Planta RJ

Abstract

The primary structure of the genes encoding the yeast ribosomal proteins L17a and L25 was determined, as well as the positions of the 5'- and 3'-termini of the corresponding mRNAs. Comparison of the gene sequences to those obtained for various other yeast ribosomal protein genes revealed several similarities. In all split genes the intron is located near the 5'-side of the amino acid coding region. Among the introns a clear pattern of sequence conservation can be observed. In particular the intron-exon boundaries and a region close to the 3'-splice site show sequence homology. Conserved sequences were also found in the leader and trailer regions of the ribosomal protein mRNAs. The 5'-flanking regions of the yeast ribosomal protein genes appeared to contain sequence elements that many but not all ribosomal protein genes have in common, and therefore may be implicated in the coordinate expression of these genes. The amino acid coding sequences of the ribosomal protein genes show a biased codon usage. Like most yeast ribosomal protein molecules, L17a and L25 are particularly basic at their N-terminus.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Restriction Enzymes Genes Genes, Fungal Plasmids RNA, Messenger/genetics Ribosomal Proteins/genetics Saccharomyces cerevisiae/genetics
Chemicals
RNA, Messenger Ribosomal Proteins ribosomal protein L17 ribosomal protein L25 DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Leer R J
van Raamsdonk-Duin M M
Hagendoorn M J
Mager W H
Planta R J
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39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.17.6685
Published
1984-09-11
Pages
6685-700
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320109
Subset
IM
Databases
GENBANK
X01014, X01694
External Links
PubMed source DOI: 10.1093/nar/12.17.6685 PMC Full Text View journal details
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