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PMID: 6273793 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ribosomal protein genes rp 39(10 - 78), rp 39(11 - 40), rp 51, and rp 52 are not contiguous to other ribosomal protein genes in the Saccharomyces cerevisiae genome.

Nucleic acids research ·Vol. 9 ·No. 19 ·1981-10-10 ·Pages 5021-36

Woolford JL, Rosbash M

Abstract

A library of recombinant phage containing EcoR1 fragments of Saccharomyces cerevisiae DNA has been constructed. This library was screened with four different recombinant plasmids, each containing a different yeast ribosomal protein gene, in order to isolate chromosomal fragments extending in both directions from these genes. These chromosomal fragments were assayed for the presence of additional ribosomal protein genes by hybridization selection and cell free translation, and none were found. These four regions are not closely linked to each other, since DNA from one domain does not cross hybridize with DNA from any of the other three, except for the sequences within the homologous ribosomal protein 39 gene pair. Northern blots demonstrate that although the concentrations of ribosomal protein mRNAs are diminished significantly in a strain containing the ts mutation rna2, transcripts from genes in these flanking segments are relatively unaffected.

MeSH Terms
DNA Restriction Enzymes/metabolism DNA, Fungal/isolation & purification DNA, Recombinant/metabolism Deoxyribonuclease BamHI Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Electrophoresis, Polyacrylamide Gel Nucleic Acid Hybridization Plasmids RNA, Messenger/isolation & purification Ribosomal Proteins/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA, Fungal DNA, Recombinant RNA, Messenger Ribosomal Proteins DNA Restriction Enzymes Deoxyribonuclease BamHI Deoxyribonuclease EcoRI Deoxyribonuclease HindIII
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Woolford J L
Rosbash M
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.19.5021
Published
1981-10-10
Pages
5021-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327496
Subset
IM
Grants
NIGMS NIH HHS · GM23549 · United States
NIGMS NIH HHS · GM28301 · United States
NICHD NIH HHS · HD08887 · United States
External Links
PubMed source DOI: 10.1093/nar/9.19.5021 PMC Full Text View journal details
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