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PMID: 7048308 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Construction of a yeast actin gene intron deletion mutant that is defective in splicing and leads to the accumulation of precursor RNA in transformed yeast cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 11 ·1982-06-00 ·Pages 3493-7

Gallwitz D

Abstract

The actin gene in yeast Saccharomyces cerevisiae is interrupted by a 309-base-pair intron within the protein-coding region. By using nuclease BAL-31, several intron deletion mutants were constructed to define sequences at the 5' splice junction that are required for RNA splicing. Extensive parts of the intron can be removed without affecting correct splicing. One mutant gene from which the invariant thymidine residue in the second intron position was deleted led to the accumulation of large amounts of unspliced actin mRNA when introduced into yeast cells through a recombinant high-copy-number plasmid. No evidence for the usage of alternative splice sites was obtained.

MeSH Terms
Actins/genetics Base Sequence Chromosome Deletion Genes Mutation Nucleic Acid Precursors/metabolism RNA Splicing RNA, Messenger/metabolism Saccharomyces cerevisiae/genetics Structure-Activity Relationship
Chemicals
Actins Nucleic Acid Precursors RNA, Messenger
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gallwitz D
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.11.3493
Published
1982-06-00
Pages
3493-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346447
Subset
IM
Databases
GENBANK
J01311
External Links
PubMed source DOI: 10.1073/pnas.79.11.3493 PMC Full Text View journal details
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