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PMID: 1741384 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 4 ·1992-02-15 ·Pages 1301-4

Mayeda A, Zahler AM, Krainer AR, Roth MB

Abstract

Monoclonal antibody 104 recognizes a subset of amphibian nuclear granules (B-snurposomes) and active sites of RNA polymerase II transcription in vertebrates and invertebrates. Monoclonal antibody 104 reacts with a set of nuclear serine- and arginine-rich phosphoproteins (SR family) with strikingly conserved apparent molecular masses. The most abundant family members in human (SRp33) and Drosophila (SRp55) cell lines can replace one another as essential splicing factors in a human cell-free system. Each of these polypeptides can functionally replace human SF2, an essential splicing factor that also regulates 5' splice site selection of alternatively spliced pre-mRNAs in vitro. Drosophila SRp55 also functions as an alternative splicing factor in the human cell-free system. Analysis of cloned cDNAs shows that SRp55 and SF2 are highly related and reveals regions of similarity to genetically defined regulators of alternative splicing in Drosophila. These results suggest that the conserved SR family of phosphoproteins, which includes SRp55 and SF2, is involved in constitutive pre-mRNA splicing and in the specificity of alternative splice site selection.

Related Genes
SF2 SRp55
MeSH Terms
Amino Acid Sequence Animals Drosophila melanogaster Humans Molecular Sequence Data Multigene Family Nuclear Proteins/isolation & purification,physiology Phosphoproteins/isolation & purification,physiology RNA Splicing RNA-Binding Proteins Sequence Alignment Serine-Arginine Splicing Factors
Chemicals
Nuclear Proteins Phosphoproteins RNA-Binding Proteins Serine-Arginine Splicing Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mayeda A
Cold Spring Harbor Laboratory, NY 11724-2208.
Zahler A M
Krainer A R
Roth M B
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.4.1301
Published
1992-02-15
Pages
1301-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48437
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.4.1301 PMC Full Text View journal details
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