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PMID: 1717489 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription.

The Journal of cell biology ·Vol. 115 ·No. 3 ·1991-11-00 ·Pages 587-96

Roth MB, Zahler AM, Stolk JA

Abstract

An antibody was identified previously that recognizes sites of polymerase II transcription on lampbrush chromosomes, puffs on polytene chromosomes, and many small granules in the nucleoplasm of all cells tested. This antibody binds a conserved family of phosphorylated polypeptides in vertebrate and invertebrate cells. We developed a method for purifying these proteins that involves differential solubility in MgCl2. We isolated a Drosophila cDNA encoding one of the proteins using information obtained from microsequencing. In vivo expression studies show that this protein is concentrated on sites of polymerase II transcription and that it is highly phosphorylated. The protein shares a high degree of homology with proteins involved in alternative splicing of pre-mRNA suggesting the possibility that this protein plays a role in pre-mRNA splicing.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Base Sequence DNA/genetics DNA Polymerase II/metabolism Drosophila/genetics Epitopes/analysis Female HeLa Cells Humans Molecular Sequence Data Nucleoproteins/analysis,genetics Oocytes/physiology Phosphoproteins/analysis,genetics Polymerase Chain Reaction/methods Protein Biosynthesis Recombinant Proteins/analysis Transcription, Genetic Xenopus
Chemicals
Antibodies, Monoclonal Epitopes Nucleoproteins Phosphoproteins Recombinant Proteins DNA DNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roth M B
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Zahler A M
Stolk J A
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34 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.115.3.587
Published
1991-11-00
Pages
587-96
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289186
Subset
IM
Grants
PHS HHS · 42786-02 · United States
NCI NIH HHS · P32-CA09437 · United States
Databases
GENBANK
M60692, M60693, M60694, M60695, M60696, M60697, M63596, S56633, S59924, S66765, X58720
External Links
PubMed source DOI: 10.1083/jcb.115.3.587 PMC Full Text View journal details
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