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PMID: 1387202 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene.

Nucleic acids research ·Vol. 20 ·No. 15 ·1992-08-11 ·Pages 4009-13

Kao HY, Siliciano PG

Abstract

The product of the yeast SNP1 gene has high homology to two domains of the metazoan U1 snRNP protein 70K, which binds to stem/loop I of the U1 RNA. However, the absence of other domains conserved in metazoan 70K and the minimal effect of yeast U1 RNA stem/loop I deletion make the assignment of SNP1 as yeast 70K less clear. To address this question, we have expressed the SNP1 gene as a fusion protein in E. coli and developed a gel shift assay for U1 RNA binding. We show here that the product of the yeast SNP1 gene binds directly and specifically to the first 47 nucleotides of yeast U1 RNA, which include the stem/loop 1 structure. We therefore conclude that the SNP1 gene product is the yeast 70K homolog. This is the first yeast protein to be identified as a homolog of a metazoan snRNP protein.

Related Genes
SNP1
MeSH Terms
Escherichia coli/genetics Fungal Proteins/genetics,metabolism Molecular Probe Techniques Plasmids/genetics RNA, Small Nuclear/metabolism Recombinant Fusion Proteins/genetics,metabolism Ribonucleoproteins/genetics,metabolism Ribonucleoproteins, Small Nuclear Sequence Homology, Nucleic Acid Yeasts/genetics
Chemicals
Fungal Proteins RNA, Small Nuclear Recombinant Fusion Proteins Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kao H Y
Department of Biochemistry, University of Minnesota Medical School, Minneapolis 55455.
Siliciano P G
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.15.4009
Published
1992-08-11
Pages
4009-13
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334080
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.15.4009 PMC Full Text View journal details
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