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PMID: 7644471 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The yeast nuclear import receptor is required for mitosis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 17 ·1995-08-15 ·Pages 7647-51

Loeb JD, Schlenstedt G, Pellman D, Kornitzer D, Silver PA, Fink GR

Abstract

The nuclear import system is highly conserved among eukaryotes. Here we report the effects of a conditional mutation in SRP1, which encodes a Saccharomyces cerevisiae homolog of the vertebrate nuclear import receptor importin. Importin was isolated as a factor required for the initial targeting step of a nuclear import substrate to the nuclear envelope in a mammalian in vitro assay. We show that yeast Srp1 is similarly required for protein import. In addition, Srp1 is also required for the execution of mitosis: we demonstrate that cells containing a conditional mutation of SRP1 arrest with a G2/M phenotype in a manner analogous to classic cdc mutants. This defect may be due to the failure of the mutant to degrade the mitotic cyclin Clb2 and other proteins required for mitosis. The requirement of a nuclear import receptor for cell cycle-regulated proteolysis implies that import of cell cycle regulators into the nucleus is critical for cell cycle progression.

MeSH Terms
Animals Base Sequence Cell Nucleus/physiology DNA Primers Genes, Fungal Genes, Lethal Genotype Karyopherins Mating Factor Mitosis Molecular Sequence Data Mutagenesis Nuclear Proteins/genetics,metabolism Peptides/metabolism Pheromones/metabolism Polymerase Chain Reaction Recombinant Fusion Proteins/biosynthesis Saccharomyces cerevisiae/genetics,growth & development,metabolism Vertebrates alpha Karyopherins
Chemicals
DNA Primers Karyopherins Nuclear Proteins Peptides Pheromones Recombinant Fusion Proteins alpha Karyopherins Mating Factor
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Loeb J D
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142, USA.
Schlenstedt G
Pellman D
Kornitzer D
Silver P A
Fink G R
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.17.7647
Published
1995-08-15
Pages
7647-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41202
Subset
IM
Grants
NIGMS NIH HHS · GM-36373 · United States
NIGMS NIH HHS · GM-40266 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.17.7647 PMC Full Text View journal details
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