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PMID: 12628931 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

High-resolution sperm typing of meiotic recombination in the mouse MHC Ebeta gene.

The EMBO journal ·Vol. 22 ·No. 6 ·2003-03-17 ·Pages 1389-97

Yauk CL, Bois PR, Jeffreys AJ

Abstract

Meiotic crossovers detected by pedigree analysis in the mouse MHC cluster into hotspots. To explore the properties of hotspots, we subjected the class II E(beta) gene to high-resolution sperm crossover analysis. We confirm the presence of a highly localized hotspot 1.0-1.6 kb wide in the second intron of E(beta) and show that it is flanked by DNA which is almost completely recombinationally inert. Mice heterozygous for haplotype s and another MHC haplotype show major haplotype-dependant variation in crossover rate but always the same hotspot, even in crosses including the highly diverged p haplotype. Crossovers in reciprocal orientations occur at similar rates but show different distributions across the hotspot, with the position of centre points in the two orientations shifted on average by 400 bp. This asymmetry results in crossover products showing biased gene conversion in favour of hotspot markers from the non-initiating haplotype, and supports the double-strand break repair model of recombination, with haplotype s as the most efficient crossover initiator. The detailed behaviour of the E(beta) hotspot, including evidence for highly localized recombination initiation, is strikingly similar to human hotspots.

MeSH Terms
Alleles Animals Base Sequence Crossing Over, Genetic Genes, MHC Class II Genetic Markers Genetic Variation Haplotypes Introns Major Histocompatibility Complex Male Meiosis/genetics Mice Mice, Inbred C57BL Mice, Inbred Strains Polymorphism, Single Nucleotide Recombination, Genetic Spermatozoa/metabolism
Chemicals
Genetic Markers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yauk C L
Department of Genetics, University of Leicester, Leicester, LE1 7RH, UK. [email protected]
Bois P R J
Jeffreys A J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg136
Email
[email protected]
Published
2003-03-17
Pages
1389-97
Language
English
Region
England
NLM ID
8208664
PMCID
PMC151079
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg136 PMC Full Text View journal details
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