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PMID: 2895998 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular characterization of meiotic recombination within the major histocompatibility complex of the mouse: mapping of crossover sites within the I region.

Biochemical genetics ·Vol. 25 ·No. 7-8 ·1987-08-00 ·Pages 513-26

Passmore HC, Kobori JA, Zimmerer EJ, Spinella DG, Hood L

Abstract

The molecular analysis of crossing-over within the mouse major histocompatibility complex provides a useful approach for the study of the structural characteristics of meiotic recombination. In this study five intra-I-region recombinants, each derived from Ik/Ib heterozygotes, were characterized for restriction-fragment length polymorphisms (RFLPs) characteristic of the I region of the two parental strains. Southern blot analysis of intra-I recombinant strains A.TBR2, A.TBR3, A.TBR5, A.TBR13, and A.TBR17 using six I-region DNA probes revealed that the point of crossing-over in all five recombinants occurred within a 6.2-kb KpnI-EcoRI segment located within the E beta gene. The segments of DNA containing the crossover point from each of the recombinant chromosomes were cloned by screening partial genomic libraries constructed in lambda gt7 bacteriophage. Construction of partial restriction maps of the cloned segments from the parental and recombinant chromosomes permitted the boundaries of the area containing the crossover site to be narrowed to a 4.0-kb segment located almost entirely within an intron of the E beta gene. The recognition that the points of crossing-over in all five recombinants studied are clustered in a relatively small area of the I region provides further evidence for a hot spot of recombination associated with the E beta gene.

MeSH Terms
Animals Chromosome Mapping Crossing Over, Genetic Genes, MHC Class I Meiosis Mice Mice, Inbred C57BL Mice, Inbred Strains Nucleic Acid Hybridization Polymorphism, Restriction Fragment Length Recombination, Genetic
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Passmore H C
Division of Biology, California Institute of Technology, Pasadena 91125.
Kobori J A
Zimmerer E J
Spinella D G
Hood L
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Article Info
Journal
Biochemical genetics
Abbr.
Biochem Genet
ISSN
0006-2928
DOI
10.1007/BF00554353
Published
1987-08-00
Pages
513-26
Language
English
Region
United States
NLM ID
0126611
Subset
IM
Grants
NIAID NIH HHS · AI14424 · United States
NIAID NIH HHS · AI20317 · United States
External Links
PubMed source DOI: 10.1007/BF00554353 View journal details
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