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PMID: 3011948 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mapping of a second recombination hot spot within the I-E region of the mouse H-2 gene complex.

The Journal of experimental medicine ·Vol. 163 ·No. 6 ·1986-06-01 ·Pages 1518-28

Lafuse WP, Berg N, Savarirayan S, David CS

Abstract

The crossover points of nine intra-I region recombinant mouse strains were determined by restriction fragment analysis. The recombinants were examined for the presence of k and p haplotype specific DNA restriction endonuclease sites. These restriction sites were a Sac I site between the E beta and E beta 2 genes, a Hpa I site within the E beta 2 gene, and a Rsa I site approximately 1 kb to the right of the E alpha gene. Seven of the recombinants were found to have crossovers between the Hpa I and the Rsa I site. This analysis suggests that a recombination hot spot exists within this segment. This segment is approximately 12-14 kb, and contains the E alpha gene and the intervening sequence between the E beta 2 and E alpha genes.

MeSH Terms
Animals DNA/analysis DNA Restriction Enzymes H-2 Antigens/genetics Histocompatibility Antigens/genetics Histocompatibility Antigens Class II Major Histocompatibility Complex Mice Oligodeoxyribonucleotides/analysis Polymorphism, Genetic Recombination, Genetic
Chemicals
H-2 Antigens Histocompatibility Antigens Histocompatibility Antigens Class II I-E-antigen Oligodeoxyribonucleotides DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lafuse W P
Berg N
Savarirayan S
David C S
References (21)
21 references, click to expand
  1. Evidence for the expression of Ia (H-2-associated) antigens on thymus-derived lymphocytes.
    J Exp Med. 1974 Nov 1;140(5):1273-84 PMID: 4547437
  2. Molecular characterization of the recombination region of six murine major histocompatibility complex (MHC) I-region recombinants.
    J Mol Cell Immunol. 1984;1(2):125-35 PMID: 6152893
  3. Chi, a promoter of generalized recombination in lambda phage, is present in immunoglobulin genes.
    Nature. 1981 Oct 1;293(5831):402-4 PMID: 6456417
  4. Polymorphic DNA region adjacent to the 5' end of the human insulin gene.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5759-63 PMID: 6272317
  5. Duplication/deletion polymorphism 5' - to the human beta globin gene.
    Nucleic Acids Res. 1981 Oct 10;9(19):5037-47 PMID: 7312624
  6. Variation in the sequence and modification state of the human insulin gene flanking regions.
    Nucleic Acids Res. 1982 Apr 10;10(7):2225-40 PMID: 6283472
  7. A molecular map of the immune response region from the major histocompatibility complex of the mouse.
    Nature. 1982 Nov 4;300(5887):35-42 PMID: 6290895
  8. A new wild-derived H-2 haplotype enhancing K-IA recombination.
    Nature. 1982 Nov 25;300(5890):370-2 PMID: 6815537
  9. DNA sequence of the gene encoding the E alpha Ia polypeptide of the BALB/c mouse.
    Science. 1982 Dec 17;218(4578):1229-32 PMID: 6815800
  10. Several mechanisms can account for defective E alpha gene expression in different mouse haplotypes.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):273-7 PMID: 6296871
  11. The murine E alpha immune response gene.
    Cell. 1983 Mar;32(3):745-54 PMID: 6403249
  12. Recombination within and between the human insulin and beta-globin gene loci.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4808-12 PMID: 6348773
  13. Structure of the murine immune response I-A beta locus: sequence of the I-A beta gene and an adjacent beta-chain second domain exon.
    Cell. 1983 Aug;34(1):179-88 PMID: 6411350
  14. Complete primary structures of the E beta chain and gene of the mouse major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5520-4 PMID: 6310581
  15. The interaction of cos with Chi is separable from DNA packaging in recA-recBC-mediated recombination of bacteriophage lambda.
    Genetics. 1983 Aug;104(4):549-70 PMID: 6225696
  16. Genes of the major histocompatibility complex in mouse and man.
    Science. 1983 Nov 18;222(4625):727-33 PMID: 6356354
  17. The nucleotide sequence of the murine I-E beta b immune response gene: evidence for gene conversion events in class II genes of the major histocompatibility complex.
    EMBO J. 1984 Jun;3(6):1221-5 PMID: 6086309
  18. Eo: a history of a mutation.
    EMBO J. 1984 Jul;3(7):1647-54 PMID: 6745246
  19. Tracts of high or low sequence divergence in the mouse major histocompatibility complex.
    EMBO J. 1984 Dec 1;3(12):2995-3003 PMID: 6098460
  20. The mutation and polymorphism of the human beta-globin gene and its surrounding DNA.
    Annu Rev Genet. 1984;18:131-71 PMID: 6084979
  21. Special sites in generalized recombination.
    Annu Rev Genet. 1979;13:7-24 PMID: 395896
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.163.6.1518
Published
1986-06-01
Pages
1518-28
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188118
Subset
IM
Grants
NIAID NIH HHS · AI-14764 · United States
NCI NIH HHS · CA-24473 · United States
External Links
PubMed source DOI: 10.1084/jem.163.6.1518 PMC Full Text View journal details
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