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PMID: 6348773 Published · ppublish English Journal Article

Recombination within and between the human insulin and beta-globin gene loci.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 15 ·1983-08-00 ·Pages 4808-12

Lebo RV, Chakravarti A, Buetow KH, Cheung MC, Cann H, Cordell B, Goodman H

Abstract

We detected a large number of polymorphic insulin restriction fragments in black Americans. These different size fragments were probably generated by unequal recombination on both sides of the human insulin gene. Population genetic analysis indicates that recombination occurred 33 times more frequently than expected to generate this large number of polymorphic fragments. Specific properties of the unique repeated 14- to 16-base-pair sequences 5' to the insulin gene suggest that this sequence would promote increased unequal recombination. Additional pedigree analysis showed that the recombination rate between the structural insulin and beta-globin gene loci was 14% with strong evidence for linkage. Since both insulin and beta-globin have been mapped to the short arm of human chromosome 11, this study establishes that the genetic map distance between these genes is 14.2 centimorgans.

MeSH Terms
Base Composition Base Sequence Blacks DNA/isolation & purification Electrophoresis, Cellulose Acetate Female Genes Genetic Variation Globins/genetics Humans Insulin/genetics Male Pedigree Polymorphism, Genetic Recombination, Genetic United States
Chemicals
Insulin Globins DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lebo R V
Chakravarti A
Buetow K H
Cheung M C
Cann H
Cordell B
Goodman H
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.15.4808
Published
1983-08-00
Pages
4808-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384134
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.15.4808 PMC Full Text View journal details
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