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PMID: 6225696 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The interaction of cos with Chi is separable from DNA packaging in recA-recBC-mediated recombination of bacteriophage lambda.

Genetics ·Vol. 104 ·No. 4 ·1983-08-00 ·Pages 549-70

Kobayashi I, Stahl MM, Leach D, Stahl FW

Abstract

Chi (5'-GCTGGTGG) is a recombinator in RecA-RecBC-mediated recombination in Escherichia coli. In bacteriophage lambda vegetative recombination, Chi is fully active only when it is correctly oriented with respect to cos, the site that defines the ends of the packaged chromosome. Here we demonstrate that packaging from cos is not necessary for this cos-Chi interaction. Our evidence suggests that correctly oriented cos is an activator of Chi. cos, as an activator, is (1) dominant over cos-, (2) active opposite an extensive heterology, (3) able to interact with Chi only when on the same (cis) chromosome, and (4) able to interact with Chi at distances as far as greater than or equal to 20 kb. Thus, cos and Chi form a two-component recombinator system for general recombination. cos may serve as an asymmetric entry site for a recombination enzyme that recognizes Chi in an asymmetric way.

MeSH Terms
Bacteriophage lambda/genetics Crossing Over, Genetic DNA, Viral/genetics Escherichia coli/genetics Recombination, Genetic
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kobayashi I
Stahl M M
Leach D
Stahl F W
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29 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/104.4.549
Published
1983-08-00
Pages
549-70
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1202126
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/104.4.549 PMC Full Text View journal details
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