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PMID: 12649325 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Global and specific transcriptional repression by the histone H3 amino terminus in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 7 ·2003-04-01 ·Pages 4084-9

Sabet N, Tong F, Madigan JP, Volo S, Smith MM, Morse RH

Abstract

The yeast CHA1 promoter is activated in the presence of serine or threonine. Activation requires the Cha4p activator, and it results in perturbation of a nucleosome that incorporates the TATA element under noninducing conditions. We show that in yeast lacking the amino terminus of histone H3, the promoter is constitutively active and the chromatin is concomitantly perturbed. This derepression occurs in the absence of elevated intracellular levels of serine or threonine and is not observed in cells lacking Rpd3p, Tup1p, or the amino terminus of histone H4. Furthermore, derepression in the absence of the H3 amino terminus requires the primary activator of this promoter, Cha4p, which we show by chromatin immunoprecipitation to be constitutively bound to the CHA1 promoter in WT yeast. Thus, the H3 amino terminus is required to prevent Cha4p from activating CHA1 in the absence of inducer. We also present results of a microarray experiment showing that the H3 amino terminus has a substantial repressive effect on a genome-wide scale.

MeSH Terms
Amino Acids/metabolism Gene Expression Regulation, Fungal Histones/chemistry,genetics Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins/chemistry,genetics Sequence Deletion TATA Box Transcription, Genetic
Chemicals
Amino Acids Histones Saccharomyces cerevisiae Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sabet Nevin
Laboratory of Developmental Genetics, Wadsworth Center, New York State Department of Health, Albany, NY 12201-2002, USA.
Tong Fumin
Madigan James P
Volo Sam
Smith M Mitchell
Morse Randall H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0637524100
Published
2003-04-01
Epub
2003-00-20
Pages
4084-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC153052
Subset
IM
Grants
NIGMS NIH HHS · R01 GM028920 · United States
NIGMS NIH HHS · R01 GM051993 · United States
NIGMS NIH HHS · GM28920 · United States
NIGMS NIH HHS · GM51993 · United States
External Links
PubMed source DOI: 10.1073/pnas.0637524100 PMC Full Text View journal details
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