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PMID: 9303316 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Role of histone tails in nucleosome remodeling by Drosophila NURF.

The EMBO journal ·Vol. 16 ·No. 15 ·1997-08-01 ·Pages 4717-26

Georgel PT, Tsukiyama T, Wu C

Abstract

The Drosophila nucleosome remodeling factor NURF utilizes the energy of ATP hydrolysis to perturb the structure of nucleosomes and facilitate binding of transcription factors. The ATPase activity of purified NURF is stimulated significantly more by nucleosomes than by naked DNA or histones alone, suggesting that NURF is able to recognize specific features of the nucleosome. Here, we show that the interaction between NURF and nucleosomes is impaired by proteolytic removal of the N-terminal histone tails and by chemical cross-linking of nucleosomal histones. The ATPase activity of NURF is also competitively inhibited by each of the four Drosophila histone tails expressed as GST fusion proteins. A similar inhibition is observed for a histone H4 tail substituted with glutamine at four conserved, acetylatable lysines. These findings indicate a novel role for the flexible histone tails in chromatin remodeling by NURF, and this role may, in part, be independent of histone acetylation.

MeSH Terms
Acetylation Adenosine Triphosphatases/antagonists & inhibitors Adenosine Triphosphate/metabolism Animals Base Sequence Cross-Linking Reagents DNA/metabolism Drosophila/genetics,metabolism Histones/chemistry,genetics,metabolism Nucleosomes/metabolism Oligodeoxyribonucleotides/genetics Recombinant Fusion Proteins/chemistry,genetics,metabolism
Chemicals
Cross-Linking Reagents Histones Nucleosomes Oligodeoxyribonucleotides Recombinant Fusion Proteins Adenosine Triphosphate DNA Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Georgel P T
Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.
Tsukiyama T
Wu C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.15.4717
Published
1997-08-01
Pages
4717-26
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170098
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.15.4717 PMC Full Text View journal details
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