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PMID: 12655003 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A non-redundant microarray of genes for two related bacteria.

Nucleic acids research ·Vol. 31 ·No. 7 ·2003-04-01 ·Pages 1869-76

Porwollik S, Frye J, Florea LD, Blackmer F, McClelland M

Abstract

A microarray with sequences from the annotated open reading frames (ORFs) in Salmonella enterica subspecies 1, serovar Typhimurium was supplemented with annotated chromosomal ORFs from serovar Typhi that are divergent from Typhimurium (>10% DNA sequence divergence). This non- redundant array was used to (i) measure changes in gene copy number in DNA from actively growing versus stationary Typhi and (ii) to reveal the transcriptional response of Typhi to peroxide, a stress similar to that experienced when they are phagocytosed by macrophages. In S.enterica subspecies 1, pairs of genomes differ in the presence or absence of approximately 10% of their genes. An array twice the size of that needed to cover all ORFs for one genome could carry close homologs of all the ORFs for 10 genomes. Non-redundant DNA arrays could be constructed for any group of closely related organisms that differ by the presence and absence of a few genes.

MeSH Terms
Gene Expression Profiling Gene Expression Regulation, Bacterial/drug effects Genes, Bacterial/genetics Oligonucleotide Array Sequence Analysis/methods Peroxides/pharmacology Salmonella enterica/drug effects,genetics Species Specificity
Chemicals
Peroxides
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Porwollik Steffen
Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.
Frye Jonathan
Florea Liliana D
Blackmer Felisa
McClelland Michael
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkg298
Published
2003-04-01
Pages
1869-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC152813
Subset
IM
Grants
NIAID NIH HHS · R01 AI034829 · United States
NIAID NIH HHS · AI34829 · United States
External Links
PubMed source DOI: 10.1093/nar/gkg298 PMC Full Text View journal details
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