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PMID: 6187001 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 3 ·1983-02-00 ·Pages 653-7

von Gabain A, Belasco JG, Schottel JL, Chang AC, Cohen SN

Abstract

An assay was developed to investigate the fate of specific segments of beta-lactamase (bla) and ompA gene transcripts in Escherichia coli. DNA probes cloned in bacteriophage M13 were treated with an endonuclease capable of cleaving single-stranded DNA, the fragments produced were annealed with total cellular RNA, and the resulting RNA . DNA hybrids were subjected to S1 nuclease treatment and gel fractionation. By using this assay, direct evidence was obtained for 3'-to-5' directionality in the decay of the long-lived mRNA encoded by the ompA gene, and no preferential stability was observed for translated versus untranslated mRNA segments. In the case of bla mRNA, initial cleavage of the full-length transcript was rate limiting, and no decay intermediates were detected. No difference in degradation rate was seen for bla transcripts having variant 3' or 5' termini.

MeSH Terms
Bacterial Outer Membrane Proteins Base Sequence Chromosome Mapping Escherichia coli/genetics Gene Expression Regulation Kinetics Membrane Proteins/genetics RNA, Bacterial/genetics RNA, Messenger/genetics,metabolism Transcription, Genetic beta-Lactamases/genetics
Chemicals
Bacterial Outer Membrane Proteins Membrane Proteins RNA, Bacterial RNA, Messenger beta-Lactamases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
von Gabain A
Belasco J G
Schottel J L
Chang A C
Cohen S N
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.3.653
Published
1983-02-00
Pages
653-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393437
Subset
IM
Grants
NIGMS NIH HHS · GM26355 · United States
NIGMS NIH HHS · GM27241 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.3.653 PMC Full Text View journal details
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