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PMID: 6253897 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cleavage of DNA.RNA hybrids by type II restriction enzymes.

Nucleic acids research ·Vol. 8 ·No. 13 ·1980-07-11 ·Pages 2939-46

Molloy PL, Symons RH

Abstract

The action of a number of restriction enzymes on DNA.RNA hybrids has been examined using hybrids synthesised with RNAs of cucumber mosaic virus as templates. The enzymes EcoRI, HindII, SalI, MspI, HhaI, AluI, TaqI and HaeIII cleaved the DNA strand of the hybrids (and possible also the RNA strand) into specific fragments. For four of these enzymes, HhaI, AluI, TaqI and HaeIII, comparison of the restriction fragments produced with the known sequences of the viral RNAs confirmed that they were recognising and cleaving the DNA strand of the hybrids at their correct recognition sequences. It is likely that the ability to utilise DNA.RNA hybrids as substrates is a general property of Type II restriction enzymes.

MeSH Terms
DNA Restriction Enzymes/metabolism DNA, Viral Molecular Weight Mosaic Viruses/analysis Nucleic Acid Hybridization RNA, Viral
Chemicals
DNA, Viral RNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Molloy P L
Symons R H
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.13.2939
Published
1980-07-11
Pages
2939-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324136
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.13.2939 PMC Full Text View journal details
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