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PMID: 12678558 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana.

Plant molecular biology ·Vol. 51 ·No. 5 ·2003-03-00 ·Pages 703-18

van Leeuwen H, Monfort A, Zhang HB, Puigdomènech P

Abstract

A bacterial artificial chromosome (BAC) library from the dihaploid melon line 'PIT92' was constructed with a 6 times coverage of the haploid melon genome. A contig of four BACs around the MRGH63 resistance gene homologue fragment was created. The complete sequence of a 117-kb BAC clone allowed to determine two clearly defined regions, the first one containing a cluster of three resistance gene homologues. Separated by a retrotransposon, that contains large long terminal repeats, the second region presents a group of genes with a conserved distribution in two regions of the Arabidopsis genome. The detailed analysis of this region provides a description of the gene structure and the presence of repetitive sequences in a defined fragment of the genome of Cucumis melo.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Base Sequence Chromosomes, Artificial, Bacterial/genetics Chromosomes, Plant/genetics Cloning, Molecular Cucumis/genetics DNA, Plant/chemistry,genetics Genes, Plant/genetics Genome, Plant Genomic Library Immunity, Innate/genetics Molecular Sequence Data Multigene Family/genetics Repetitive Sequences, Nucleic Acid/genetics Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Synteny
Chemicals
DNA, Plant
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
van Leeuwen Hans
Department de Genètica Molecular, Institut de Biologia Molecular de Barcelona, CID-CSIC, Jordi Girona 18, 08034, Barcelona, Spain.
Monfort Amparo
Zhang Hong-Bin
Puigdomènech Pere
References (45)
45 references, click to expand
  1. Plant rac proteins induce superoxide production in mammalian cells.
    Biochem Biophys Res Commun. 2000 Jun 16;272(3):783-8 PMID: 10860831
  2. Splice site prediction in Arabidopsis thaliana pre-mRNA by combining local and global sequence information.
    Nucleic Acids Res. 1996 Sep 1;24(17):3439-52 PMID: 8811101
  3. Plant genome evolution: lessons from comparative genomics at the DNA level.
    Plant Mol Biol. 2002 Jan;48(1-2):21-37 PMID: 11860210
  4. Unequal exchange and meiotic instability of disease-resistance genes in the Rp1 region of maize.
    Genetics. 1993 Jan;133(1):119-25 PMID: 8417982
  5. Novel properties of satellite DNA from muskmelon.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1511-5 PMID: 4524654
  6. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  7. Construction of a reference linkage map for melon.
    Genome. 2001 Oct;44(5):836-45 PMID: 11681608
  8. Point mutants of Moloney murine leukemia virus that fail to package viral RNA: evidence for specific RNA recognition by a "zinc finger-like" protein sequence.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8420-4 PMID: 3141927
  9. Analysis of donor splice sites in different eukaryotic organisms.
    J Mol Evol. 1997 Jul;45(1):50-9 PMID: 9211734
  10. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence.
    J Mol Biol. 2000 Jul 21;300(4):1005-16 PMID: 10891285
  11. The evolution of disease resistance genes.
    Plant Mol Biol. 2000 Jan;42(1):195-204 PMID: 10688137
  12. Xa21D encodes a receptor-like molecule with a leucine-rich repeat domain that determines race-specific recognition and is subject to adaptive evolution.
    Plant Cell. 1998 May;10(5):765-79 PMID: 9596635
  13. Early elicitor induction in members of a novel multigene family coding for highly related RING-H2 proteins in Arabidopsis thaliana.
    Plant Mol Biol. 1999 Jul;40(4):579-90 PMID: 10480382
  14. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential.
    Genome Res. 2001 Aug;11(8):1441-52 PMID: 11483586
  15. The complete sequence of 340 kb of DNA around the rice Adh1-adh2 region reveals interrupted colinearity with maize chromosome 4.
    Plant Cell. 2000 Mar;12(3):381-91 PMID: 10715324
  16. BCM Search Launcher--an integrated interface to molecular biology data base search and analysis services available on the World Wide Web.
    Genome Res. 1996 May;6(5):454-62 PMID: 8743995
  17. Isolation and analysis of expression mechanisms of a rice gene, EL5, which shows structural similarity to ATL family from Arabidopsis, in response to N-acetylchitooligosaccharide elicitor.
    Plant Sci. 2001 Mar;160(4):577-583 PMID: 11448732
  18. cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles.
    Plant Cell. 2000 Jun;12(6):963-77 PMID: 10852940
  19. Conservation of microstructure between a sequenced region of the genome of rice and multiple segments of the genome of Arabidopsis thaliana.
    Genome Res. 2001 Jul;11(7):1167-74 PMID: 11435398
  20. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  21. Novel disease resistance specificities result from sequence exchange between tandemly repeated genes at the Cf-4/9 locus of tomato.
    Cell. 1997 Dec 12;91(6):821-32 PMID: 9413991
  22. Plant retrotransposon from Lilium henryi is related to Ty3 of yeast and the gypsy group of Drosophila.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):5015-9 PMID: 2544887
  23. EMBOSS: the European Molecular Biology Open Software Suite.
    Trends Genet. 2000 Jun;16(6):276-7 PMID: 10827456
  24. The InterPro database, an integrated documentation resource for protein families, domains and functional sites.
    Nucleic Acids Res. 2001 Jan 1;29(1):37-40 PMID: 11125043
  25. Conserved cysteine and histidine residues of the avian myeloblastosis virus nucleocapsid protein are essential for viral replication but are not "zinc-binding fingers".
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7094-8 PMID: 2845395
  26. A bacterial artificial chromosome (BAC) library of Malus floribunda 821 and contig construction for positional cloning of the apple scab resistance gene Vf.
    Genome. 2001 Dec;44(6):1104-13 PMID: 11768214
  27. Prediction of complete gene structures in human genomic DNA.
    J Mol Biol. 1997 Apr 25;268(1):78-94 PMID: 9149143
  28. Interpolated Markov models for eukaryotic gene finding.
    Genomics. 1999 Jul 1;59(1):24-31 PMID: 10395796
  29. Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor.
    Nucleic Acids Res. 1994 Nov 25;22(23):4922-31 PMID: 7800481
  30. Simple sequence repeats in Cucumis mapping and map merging.
    Genome. 2000 Dec;43(6):963-74 PMID: 11195350
  31. GeneMark.hmm: new solutions for gene finding.
    Nucleic Acids Res. 1998 Feb 15;26(4):1107-15 PMID: 9461475
  32. BLAST 2 Sequences, a new tool for comparing protein and nucleotide sequences.
    FEMS Microbiol Lett. 1999 May 15;174(2):247-50 PMID: 10339815
  33. Angiosperm phylogeny inferred from multiple genes as a tool for comparative biology.
    Nature. 1999 Nov 25;402(6760):402-4 PMID: 10586878
  34. Construction of a rice bacterial artificial chromosome library and identification of clones linked to the Xa-21 disease resistance locus.
    Plant J. 1995 Mar;7(3):525-33 PMID: 7757120
  35. Evolution of the rice Xa21 disease resistance gene family.
    Plant Cell. 1997 Aug;9(8):1279-87 PMID: 9286106
  36. Retrotransposon evolution in diverse plant genomes.
    Genetics. 2000 Sep;156(1):313-25 PMID: 10978295
  37. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
    Nature. 2000 Dec 14;408(6814):796-815 PMID: 11130711
  38. The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins.
    Cell. 1996 Feb 9;84(3):451-9 PMID: 8608599
  39. Marked improvement of PAC and BAC cloning is achieved using electroelution of pulsed-field gel-separated partial digests of genomic DNA.
    Nucleic Acids Res. 1997 Oct 1;25(19):3959-61 PMID: 9380525
  40. Comparing sequenced segments of the tomato and Arabidopsis genomes: large-scale duplication followed by selective gene loss creates a network of synteny.
    Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):9121-6 PMID: 10908680
  41. Distribution of microsatellites in relation to coding sequences within the Arabidopsis thaliana genome.
    Plant Sci. 2000 Aug 8;157(1):97-104 PMID: 10940473
  42. The Staden sequence analysis package.
    Mol Biotechnol. 1996 Jun;5(3):233-41 PMID: 8837029
  43. Recent improvements to the SMART domain-based sequence annotation resource.
    Nucleic Acids Res. 2002 Jan 1;30(1):242-4 PMID: 11752305
  44. Comparative sequence analysis reveals extensive microcolinearity in the lateral suppressor regions of the tomato, Arabidopsis, and Capsella genomes.
    Plant Cell. 2001 Apr;13(4):979-88 PMID: 11283350
  45. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1022573230486
Published
2003-03-00
Pages
703-18
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
AF499727
External Links
PubMed source DOI: 10.1023/a:1022573230486 View journal details
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