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PMID: 12695489 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interleukin (IL)-15R[alpha]-deficient natural killer cells survive in normal but not IL-15R[alpha]-deficient mice.

The Journal of experimental medicine ·Vol. 197 ·No. 8 ·2003-04-21 ·Pages 977-84

Koka R, Burkett PR, Chien M, Chai S, Chan F, Lodolce JP, Boone DL, Ma A

Abstract

Natural killer (NK) cells protect hosts against viral pathogens and transformed cells. IL-15 is thought to play a critical role in NK cell development, but its role in the regulation of peripheral NK cells is less well defined. We now find that adoptive transfer of normal NK cells into mice lacking the high affinity interleukin (IL)-15 receptor, IL-15Ralpha, surprisingly results in the abrupt loss of these cells. Moreover, IL-15Ralpha-deficient NK cells can differentiate successfully in radiation bone marrow chimera bearing normal cells. Finally, adoptively transferred IL-15Ralpha-deficient NK cells survive in normal but not IL-15Ralpha-deficient mice. These findings demonstrate that NK cell-independent IL-15Ralpha expression is critical for maintaining peripheral NK cells, while IL-15Ralpha expression on NK cells is not required for this function.

MeSH Terms
Adoptive Transfer Animals Cell Survival/physiology Homeostasis Interleukin-15/genetics,metabolism Killer Cells, Natural/cytology,metabolism Mice Mice, Inbred Strains Mice, Knockout Radiation Chimera/physiology Receptors, Interleukin-15 Receptors, Interleukin-2/genetics,metabolism Signal Transduction/physiology Spleen/cytology
Chemicals
Il15ra protein, mouse Interleukin-15 Receptors, Interleukin-15 Receptors, Interleukin-2
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Koka Rima
Department of Medicine, University of Chicago, 5841 S. Maryland Ave., MC6084, Chicago, IL 60637, USA.
Burkett Patrick R
Chien Marcia
Chai Sophia
Chan Faye
Lodolce James P
Boone David L
Ma Averil
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20021836
Published
2003-04-21
Epub
2003-00-14
Pages
977-84
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2193874
Subset
IM
Grants
NIAID NIH HHS · AI07090 · United States
NIDDK NIH HHS · P30 DK042086 · United States
NIAID NIH HHS · AI45860 · United States
NIGMS NIH HHS · GM07281 · United States
NIAID NIH HHS · R01 AI045860 · United States
NIGMS NIH HHS · T32 GM007281 · United States
NIDDK NIH HHS · DK42086 · United States
NIAID NIH HHS · T32 AI007090 · United States
External Links
PubMed source DOI: 10.1084/jem.20021836 PMC Full Text View journal details
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