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PMID: 12704150 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inactivation of the srtA gene affects localization of surface proteins and decreases adhesion of Streptococcus pneumoniae to human pharyngeal cells in vitro.

Infection and immunity ·Vol. 71 ·No. 5 ·2003-05-00 ·Pages 2758-65

Kharat AS, Tomasz A

Abstract

Inactivation of sortase gene srtA in Streptococcus pneumoniae strain R6 caused the release of beta-galactosidase and neuraminidase A (NanA) from the cell wall into the surrounding medium. Both of these surface proteins contain the LPXTG motif in the C-terminal domain. Complementation with plasmid-borne srtA reversed protein release. Deletion of murM, a gene involved in the branching of pneumococcal peptidoglycan, also caused partial release of beta-galactosidase, suggesting preferential attachment of the protein to branched muropeptides in the cell wall. Inactivation of srtA caused decreased adherence to human pharyngeal cells in vitro but had no effect on the virulence of a capsular type III strain of S. pneumoniae in the mouse intraperitoneal model. The observations suggest that--as in other gram-positive bacteria--sortase-dependent display of proteins occurs in S. pneumoniae and that some of these proteins may be involved in colonization of the human host.

MeSH Terms
Amino Acid Motifs Amino Acid Sequence Aminoacyltransferases/genetics,physiology Animals Bacterial Adhesion Bacterial Proteins/analysis Cell Line Cysteine Endopeptidases Humans Membrane Proteins/analysis Mice Molecular Sequence Data Neuraminidase/analysis Pharynx/microbiology Streptococcus pneumoniae/genetics,physiology Virulence beta-Galactosidase/analysis
Chemicals
Bacterial Proteins Membrane Proteins Aminoacyltransferases sortase A Neuraminidase beta-Galactosidase Cysteine Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kharat Arun S
Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA.
Tomasz Alexander
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.71.5.2758-2765.2003
Published
2003-05-00
Pages
2758-65
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC153252
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.71.5.2758-2765.2003 PMC Full Text View journal details
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