Studies on the biological role of DNA methylation: inhibition of methylation and maturation of the bacteriophage phichi174 by nicotinamide.

Nucleic acids research ·Vol. 2 ·No. 10 ·1975-10-00 ·Pages 1967-74

Razin A, Goren D, Friedman J

Abstract

Nicotinamide was found to be a potent inhibitor of DNA methylation in vivo without interfering with protein or DNA synthesis. The inhibition of DNA methylation in a phage-infected cell resulted in a parallel decrease in the production of viable virus particles. In vitro experiments revealed that nicotinamide inhibits DNA methylase activity in a competitive fashion with respect to S-adenosylmethionine and non-competitively with respect to DNA. These results were interpreted to mean that DNA methylation is an essential step in the process of maturation of the bacteriophage phichi174.

MeSH Terms

Coliphages/drug effects,enzymology DNA (Cytosine-5-)-Methyltransferases/antagonists & inhibitors Kinetics Methyltransferases/antagonists & inhibitors Niacinamide/pharmacology S-Adenosylmethionine/pharmacology Species Specificity

Chemicals

Niacinamide S-Adenosylmethionine Methyltransferases DNA (Cytosine-5-)-Methyltransferases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Razin A

Goren D

Friedman J

References (15)

15 references, click to expand

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In vivo methylation of replicating bacteriophage phi chi174 DNA.
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DNA modification and restriction.
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The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.
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Biochem Biophys Res Commun. 1971 Feb 19;42(4):602-7 PMID: 5543941
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/2.10.1967

Published

1975-10-00

Pages

1967-74

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343560

Subset

External Links

PubMed source DOI: 10.1093/nar/2.10.1967 PMC Full Text View journal details

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Studies on the biological role of DNA methylation: inhibition of methylation and maturation of the bacteriophage phichi174 by nicotinamide.

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