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PMID: 12724543 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid genome divergence at orthologous low molecular weight glutenin loci of the A and Am genomes of wheat.

The Plant cell ·Vol. 15 ·No. 5 ·2003-05-00 ·Pages 1186-97

Wicker T, Yahiaoui N, Guyot R, Schlagenhauf E, Liu ZD, Dubcovsky J, Keller B

Abstract

To study genome evolution in wheat, we have sequenced and compared two large physical contigs of 285 and 142 kb covering orthologous low molecular weight (LMW) glutenin loci on chromosome 1AS of a diploid wheat species (Triticum monococcum subsp monococcum) and a tetraploid wheat species (Triticum turgidum subsp durum). Sequence conservation between the two species was restricted to small regions containing the orthologous LMW glutenin genes, whereas >90% of the compared sequences were not conserved. Dramatic sequence rearrangements occurred in the regions rich in repetitive elements. Dating of long terminal repeat retrotransposon insertions revealed different insertion events occurring during the last 5.5 million years in both species. These insertions are partially responsible for the lack of homology between the intergenic regions. In addition, the gene space was conserved only partially, because different predicted genes were identified on both contigs. Duplications and deletions of large fragments that might be attributable to illegitimate recombination also have contributed to the differentiation of this region in both species. The striking differences in the intergenic landscape between the A and A(m) genomes that diverged 1 to 3 million years ago provide evidence for a dynamic and rapid genome evolution in wheat species.

MeSH Terms
Base Sequence Chromosomes, Plant/genetics Conserved Sequence/genetics Contig Mapping DNA, Intergenic/genetics Evolution, Molecular Genes, Plant/genetics Genetic Variation Genome, Plant Glutens/analogs & derivatives,genetics Molecular Sequence Data Molecular Weight Physical Chromosome Mapping Pseudogenes/genetics Species Specificity Triticum/genetics
Chemicals
DNA, Intergenic Glutens glutenin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wicker Thomas
Institute of Plant Biology, University of Zürich, Switzerland.
Yahiaoui Nabila
Guyot Romain
Schlagenhauf Edith
Liu Zhong-Da
Dubcovsky Jorge
Keller Beat
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.011023
Published
2003-05-00
Pages
1186-97
Language
English
Region
England
NLM ID
9208688
PMCID
PMC153725
Subset
IM
Databases
GENBANK
AY146587, AY146588
External Links
PubMed source DOI: 10.1105/tpc.011023 PMC Full Text View journal details
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