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PMID: 12746531 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Quantitative trait loci and comparative genomics of cereal cell wall composition.

Plant physiology ·Vol. 132 ·No. 1 ·2003-05-00 ·Pages 263-71

Hazen SP, Hawley RM, Davis GL, Henrissat B, Walton JD

Abstract

Quantitative trait loci (QTLs) affecting sugar composition of the cell walls of maize (Zea mays) pericarp were mapped as an approach to the identification of genes involved in cereal wall biosynthesis. Mapping was performed using the IBM (B73 x Mo17) recombinant inbred line population. There were statistically significant differences between B73 and Mo17 in content of xylose (Xyl), arabinose (Ara), galactose (Gal), and glucose. Thirteen QTLs were found, affecting the content of Xyl (two QTLs), Ara (two QTLs), Gal (five QTLs), Glc (two QTLs), Ara + Gal (one QTL), and Xyl + Glc (one QTL). The chromosomal regions corresponding to two of these, affecting Ara + Gal and Ara on maize chromosome 3, could be aligned with a syntenic region on rice (Oryza sativa) chromosome 1, which has been completely sequenced and annotated. The contiguous P1-derived artificial chromosome rice clones covering the QTLs were predicted to encode 117 and 125 proteins, respectively. Two of these genes encode putative glycosyltransferases, displaying similarity to carbohydrate-active enzyme database family GT4 (galactosyltransferases) or to family GT64 (C-terminal domain of animal heparan synthases). The results illustrate the potential of using natural variation, emerging genomic resources, and homeology within the Poaceae to identify candidate genes involved in the essential process of cell wall biosynthesis.

MeSH Terms
Carbohydrate Metabolism Cell Wall/metabolism Chromosome Mapping Chromosomes, Plant/genetics Genomics/methods Oryza/genetics,metabolism Poaceae/genetics,metabolism Quantitative Trait Loci/genetics Seeds/genetics,metabolism Zea mays/genetics,metabolism
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hazen Samuel P
Department of Agronomy, University of Missouri, Columbia 65211, USA.
Hawley Robin M
Davis Georgia L
Henrissat Bernard
Walton Jonathan D
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.103.020016
Published
2003-05-00
Pages
263-71
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC166971
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.103.020016 PMC Full Text View journal details
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