Thymineless bacteriophage induction in Staphylococcus aureus. I. High-frequency transduction with lysates containing a bacteriophage related to bacteriophage phi 11.

Journal of virology ·Vol. 16 ·No. 6 ·1975-12-00 ·Pages 1357-66

Rudin L, Lindberg M

Abstract

A thymine-requiring mutant of Staphylococcus aureus, strain 8325 (PI258)thy, undergoes prophage induction and lysis after thymine starvation. Four different phages were isolated from the lysate in low titers, among which was a phage designated phi 14, which differs from phage phi 11 in its immunity locus. The thymineless induced lysates of strain 8325(PI258)thy transduce the penicillinase plasmid at high frequency (10(-1), whereas transduction of chromosomal markers is inefficient. A plasmic-cured derivative of strain 8325(PI258)thy is also lysed by thymine starvation and be used for high-frequency transduction of other plasmids. Reconstitution of a strain of S. aureus that responds to thymine starvation was only partially successful, but this system can effectively be used to transduce plasmids or plasmid derivatives.

MeSH Terms

Drug Resistance, Microbial Erythromycin/pharmacology Lysogeny Mitomycins Mutation Penicillinase/biosynthesis Plasmids Staphylococcus Phages/growth & development,immunology Staphylococcus aureus/drug effects,enzymology,metabolism Thymine/metabolism Transduction, Genetic Transformation, Genetic Ultraviolet Rays Virus Replication

Chemicals

Mitomycins Erythromycin Penicillinase Thymine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Rudin L

Lindberg M

References (29)

29 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.16.6.1357-1366.1975

Published

1975-12-00

Pages

1357-66

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355743

Subset

External Links

PubMed source DOI: 10.1128/JVI.16.6.1357-1366.1975 PMC Full Text View journal details

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Thymineless bacteriophage induction in Staphylococcus aureus. I. High-frequency transduction with lysates containing a bacteriophage related to bacteriophage phi 11.

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