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PMID: 1279693 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a mouse brain cDNA that encodes a protein related to the Alzheimer disease-associated amyloid beta protein precursor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 22 ·1992-11-15 ·Pages 10758-62

Wasco W, Bupp K, Magendantz M, Gusella JF, Tanzi RE, Solomon F

Abstract

We have isolated a cDNA from a mouse brain library that encodes a protein whose predicted amino acid sequence is 42% identical and 64% similar to that of the amyloid beta protein precursor (APP). This 653-amino acid protein, which we have termed the amyloid precursor-like protein (APLP), appears to be similar to APP in overall structure as well as amino acid sequence. The amino acid homologies are concentrated within three distinct regions of the two proteins where the identities are 47%, 54%, and 56%. The APLP cDNA hybridizes to two messages of approximately 2.4 and 1.6 kilobases that are present in mouse brain and neuroblastoma cells. Polyclonal antibodies raised against a peptide derived from the C terminus of APLP stain the cytoplasm in a pattern reminiscent of Golgi staining. In addition to APP, APLP also displays significant homology to the Drosophila APP-like protein APPL and a rat testes APP-like protein. These data indicate that the APP gene is a member of a strongly conserved gene family. Studies aimed at determining the functions of the proteins encoded by this gene family should provide valuable clues to their potential role in Alzheimer disease neuropathology.

MeSH Terms
Amino Acid Sequence Amyloid beta-Protein Precursor/analogs & derivatives,analysis,genetics Animals Base Sequence Blotting, Northern Blotting, Western Brain/physiology Cloning, Molecular DNA/genetics,isolation & purification Humans Mice Microtubule-Associated Proteins/genetics Molecular Sequence Data Neuroblastoma Poly A/genetics,isolation & purification RNA/genetics,isolation & purification RNA, Messenger Recombinant Proteins/analysis,isolation & purification Restriction Mapping Sequence Homology, Amino Acid Tumor Cells, Cultured
Chemicals
APLP1 protein, human Amyloid beta-Protein Precursor Aplp1 protein, mouse Microtubule-Associated Proteins RNA, Messenger Recombinant Proteins Poly A RNA DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wasco W
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Bupp K
Magendantz M
Gusella J F
Tanzi R E
Solomon F
References (38)
38 references, click to expand
  1. A marginal band-associated protein has properties of both microtubule- and microfilament-associated proteins.
    J Cell Biol. 1989 Oct;109(4 Pt 1):1609-20 PMID: 2677023
  2. Intracellular protein topogenesis.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1496-500 PMID: 6929499
  3. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors.
    Nature. 1988 Feb 11;331(6156):525-7 PMID: 2893289
  4. Novel precursor of Alzheimer's disease amyloid protein shows protease inhibitory activity.
    Nature. 1988 Feb 11;331(6156):530-2 PMID: 2893291
  5. A simple, rapid method for the purification of poly A+ RNA.
    Biotechniques. 1988 Feb;6(2):114-6 PMID: 2908499
  6. Amyloid beta protein gene: cDNA, mRNA distribution, and genetic linkage near the Alzheimer locus.
    Science. 1987 Feb 20;235(4791):880-4 PMID: 2949367
  7. Antibodies to the conserved cytoplasmic domain of the integrin beta 1 subunit react with proteins in vertebrates, invertebrates, and fungi.
    J Cell Biol. 1988 May;106(5):1765-72 PMID: 3131349
  8. Beta-amyloid precursor protein of Alzheimer disease occurs as 110- to 135-kilodalton membrane-associated proteins in neural and nonneural tissues.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7341-5 PMID: 3140239
  9. Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin.
    Cell. 1986 Jul 18;46(2):271-82 PMID: 3487386
  10. Characterization and chromosomal localization of a cDNA encoding brain amyloid of Alzheimer's disease.
    Science. 1987 Feb 20;235(4791):877-80 PMID: 3810169
  11. Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells.
    J Biol Chem. 1985 Jun 10;260(11):6654-62 PMID: 3922977
  12. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  13. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  14. Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein.
    Proc Natl Acad Sci U S A. 1990 Apr;87(7):2405-8 PMID: 1690887
  15. The Alzheimer amyloid precursor protein. Identification of a stable intermediate in the biosynthetic/degradative pathway.
    J Biol Chem. 1990 Mar 15;265(8):4492-7 PMID: 1968460
  16. Processing of Alzheimer beta/A4 amyloid precursor protein: modulation by agents that regulate protein phosphorylation.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):6003-6 PMID: 2116015
  17. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 PMID: 2461560
  18. Structure and expression of the alternatively-spliced forms of mRNA for the mouse homolog of Alzheimer's disease amyloid beta protein precursor.
    Biochem Biophys Res Commun. 1989 Feb 15;158(3):906-12 PMID: 2493250
  19. Evidence that beta-amyloid protein in Alzheimer's disease is not derived by normal processing.
    Science. 1990 Apr 27;248(4954):492-5 PMID: 1691865
  20. NPXY, a sequence often found in cytoplasmic tails, is required for coated pit-mediated internalization of the low density lipoprotein receptor.
    J Biol Chem. 1990 Feb 25;265(6):3116-23 PMID: 1968060
  21. Cleavage of amyloid beta peptide during constitutive processing of its precursor.
    Science. 1990 Jun 1;248(4959):1122-4 PMID: 2111583
  22. Identification, secretion, and neural expression of APPL, a Drosophila protein similar to human amyloid protein precursor.
    J Neurosci. 1990 Dec;10(12):3849-61 PMID: 2125311
  23. Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2295-306 PMID: 2277061
  24. Secreted form of amyloid beta protein precursor is involved in the growth regulation of fibroblasts.
    Cell. 1989 Aug 25;58(4):615-22 PMID: 2475254
  25. A Drosophila gene encoding a protein resembling the human beta-amyloid protein precursor.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2478-82 PMID: 2494667
  26. The beta-amyloid protein precursor of Alzheimer disease has soluble derivatives found in human brain and cerebrospinal fluid.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6338-42 PMID: 2503832
  27. Identification, biogenesis, and localization of precursors of Alzheimer's disease A4 amyloid protein.
    Cell. 1989 Apr 7;57(1):115-26 PMID: 2649245
  28. A novel mRNA of the A4 amyloid precursor gene coding for a possibly secreted protein.
    Science. 1989 Aug 11;245(4918):651-3 PMID: 2569763
  29. The immunohistochemical demonstration of subsequences of the precursor of the amyloid A4 protein in senile plaques in Alzheimer's disease.
    Neuropathol Appl Neurobiol. 1989 Mar-Apr;15(2):135-47 PMID: 2657471
  30. One-sided polymerase chain reaction: the amplification of cDNA.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5673-7 PMID: 2788276
  31. Protease inhibitor domain encoded by an amyloid protein precursor mRNA associated with Alzheimer's disease.
    Nature. 1988 Feb 11;331(6156):528-30 PMID: 2893290
  32. Localization of the putative precursor of Alzheimer's disease-specific amyloid at nuclear envelopes of adult human muscle.
    EMBO J. 1988 Feb;7(2):367-72 PMID: 2896589
  33. Molecular cloning and characterization of a cDNA encoding the cerebrovascular and the neuritic plaque amyloid peptides.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):4190-4 PMID: 3035574
  34. Phosphorylation of Alzheimer disease amyloid precursor peptide by protein kinase C and Ca2+/calmodulin-dependent protein kinase II.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):6218-21 PMID: 3137567
  35. Insertions of up to 17 amino acids into a region of alpha-tubulin do not disrupt function in vivo.
    Mol Cell Biol. 1987 Oct;7(10):3799-805 PMID: 3316988
  36. Analyzing the components of microtubules: antibodies against chartins, associated proteins from cultured cells.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6581-5 PMID: 3863114
  37. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  38. The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor.
    Nature. 1987 Feb 19-25;325(6106):733-6 PMID: 2881207
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.22.10758
Published
1992-11-15
Pages
10758-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50421
Subset
IM
Grants
NCI NIH HHS · CA53395 · United States
NINDS NIH HHS · R01 NS-30428-01 · United States
Databases
GENBANK
L04538
External Links
PubMed source DOI: 10.1073/pnas.89.22.10758 PMC Full Text View journal details
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