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PMID: 12805567 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Tetraspanin CD151 regulates alpha6beta1 integrin adhesion strengthening.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 13 ·2003-06-24 ·Pages 7616-21

Lammerding J, Kazarov AR, Huang H, Lee RT, Hemler ME

Abstract

The tetraspanin CD151 molecule associates specifically with laminin-binding integrins, including alpha6beta1. To probe strength of alpha6beta1-dependent adhesion to laminin-1, defined forces (0-1.5 nN) were applied to magnetic laminin-coated microbeads bound to NIH 3T3 cells. For NIH 3T3 cells bearing wild-type CD151, adhesion strengthening was observed, as bead detachment became more difficult over time. In contrast, mutant CD151 (with the C-terminal region replaced) showed impaired adhesion strengthening. Static cell adhesion to laminin-1, and detachment of beads coated with fibronectin or anti-alpha6 antibody were all unaffected by CD151 mutation. Hence, CD151 plays a key role in selectively strengthening alpha6beta1 integrin-mediated adhesion to laminin-1.

Keywords
NASA Discipline Cell Biology Non-NASA Center
MeSH Terms
3T3 Cells Animals Antigens, CD/chemistry,genetics,physiology Calibration Cell Adhesion Cell Line Integrin alpha6beta1/metabolism Laminin/metabolism Magnetics Mice Mutation Protein Binding Protein Structure, Tertiary Stress, Mechanical Temperature Tetraspanin 24 Time Factors
Chemicals
Antigens, CD Cd151 protein, mouse Integrin alpha6beta1 Laminin Tetraspanin 24 laminin 1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lammerding Jan
Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Kazarov Alexander R
Huang Hayden
Lee Richard T
Hemler Martin E
Investigators
1 investigators, click to expand
Lee R T
Brigham & Women's Hosp, Boston, MA
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1337546100
Published
2003-06-24
Epub
2003-00-12
Pages
7616-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC164635
Subset
IM
Grants
NCI NIH HHS · R01 CA042368 · United States
NCI NIH HHS · R01-CA86712 · United States
NCI NIH HHS · R01-CA42368 · United States
NHLBI NIH HHS · P01 HL064858 · United States
NCI NIH HHS · R01 CA086712 · United States
NHLBI NIH HHS · P01-HL64858 · United States
External Links
PubMed source DOI: 10.1073/pnas.1337546100 PMC Full Text View journal details
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