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PMID: 1280641 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Genetic heterogeneity within Mycobacterium fortuitum complex species: genotypic criteria for identification.

Journal of clinical microbiology ·Vol. 30 ·No. 11 ·1992-11-00 ·Pages 2772-5

Kirschner P, Kiekenbeck M, Meissner D, Wolters J, Böttger EC

Abstract

A 1.5-kb segment of the DNA that encodes 16S rRNA was amplified by polymerase chain reaction, and 880 nucleotide positions were determined from each of the described biovariants of Mycobacterium fortuitum. Signature sequences which allow rapid identification of M. fortuitum strains at the biovariant level are described. Our data demonstrate a close phylogenetic relationship between Mycobacterium senegalense and M. fortuitum and indicate that the described biovariants of M. fortuitum represent genetically distinct taxa.

MeSH Terms
Base Sequence DNA, Bacterial/genetics Genes, Bacterial Genetic Variation Genotype Humans Molecular Sequence Data Nontuberculous Mycobacteria/classification,genetics,metabolism RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Species Specificity
Chemicals
DNA, Bacterial RNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kirschner P
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
Kiekenbeck M
Meissner D
Wolters J
Böttger E C
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.11.2772-2775.1992
Published
1992-11-00
Pages
2772-5
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC270525
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.11.2772-2775.1992 PMC Full Text View journal details
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