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PMID: 1282701 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

hnRNP A2/B1 binds specifically to single stranded vertebrate telomeric repeat TTAGGGn.

Nucleic acids research ·Vol. 20 ·No. 24 ·1992-12-25 ·Pages 6461-4

McKay SJ, Cooke H

Abstract

We have previously isolated a protein from mouse liver nuclei that specifically binds to single stranded (TTAGGG)n repeats. TTAGGG is the telomeric repeats of mammals and we therefore named the new protein single stranded telomere binding protein (sTBP). Further studies now identify sTBP as heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 on the basis of amino acid sequence determination and antibody reactivity. A2 and B1 form a major part of the protein component of hnRNP particles and are abundant nuclear proteins. Unexpectedly, A2/B1 has a high specificity for binding to the RNA equivalent of TTAGGG, UUAGGG, but under the same conditions does not appear to have a strong affinity for a number of other RNA species.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Base Sequence Binding Sites Binding, Competitive DNA/metabolism DNA-Binding Proteins/metabolism Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Immunoblotting Liver/metabolism Mice Molecular Sequence Data Nuclear Proteins/isolation & purification,metabolism Oligodeoxyribonucleotides Plasmids RNA/metabolism RNA-Binding Proteins/metabolism Repetitive Sequences, Nucleic Acid Ribonucleoproteins/immunology,isolation & purification,metabolism Vertebrates
Chemicals
Antibodies, Monoclonal DNA-Binding Proteins Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Nuclear Proteins Oligodeoxyribonucleotides RNA-Binding Proteins Ribonucleoproteins hnRNP A2 RNA DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McKay S J
Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Cooke H
References (16)
16 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.24.6461
Published
1992-12-25
Pages
6461-4
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334558
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.24.6461 PMC Full Text View journal details
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