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PMID: 3062579 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Modulation of hnRNP A1 protein gene expression by epidermal growth factor in Rat-1 cells.

Nucleic acids research ·Vol. 16 ·No. 24 ·1988-12-23 ·Pages 11663-73

Planck SR, Listerud MD, Buckley SD

Abstract

The levels of mRNA encoding hnRNP core protein A1 have been compared in quiescent and proliferating Rat-1 embryonic fibroblasts. Northern blot hybridization analyses using probes made from an A1 cDNA clone, lambda HDP-182, isolated by Cobianchi et al. (J. Biol. Chem. 261:3536-2543 (1986] indicated that three sizes of poly A + RNAs, 1.6, 2.0, & 4.0 kb, have extensive sequence homology. The levels of all three A1 RNA species are responsive to the proliferation state of the cells. Stimulation of quiescent Rat-1 cells with serum or epidermal growth factor resulted in a 2- to 5-fold increase in the levels of each of these three RNAs that was evident after 2 hours and reached a peak after about 8 hours. Addition of the protein synthesis inhibitor, cycloheximide, along with epidermal growth factor completely blocked the upsurge in A1 RNA levels. Thus, the A1 RNA species are not primary transcriptional targets of epidermal growth factor but do show an induction pattern similar to mRNAs encoding some glycolytic enzymes.

MeSH Terms
Animals Blotting, Northern Carrier Proteins/metabolism Cell Line Culture Media Cycloheximide/pharmacology Dactinomycin/pharmacology Epidermal Growth Factor/pharmacology Fibroblasts/drug effects,metabolism Gene Expression Regulation/drug effects Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Peptidylprolyl Isomerase Rats Ribonucleoproteins/genetics,metabolism Sequence Homology, Nucleic Acid
Chemicals
Carrier Proteins Culture Media Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Ribonucleoproteins Dactinomycin Epidermal Growth Factor Cycloheximide Peptidylprolyl Isomerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Planck S R
Oregon Health Sciences University, Portland 97201.
Listerud M D
Buckley S D
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.24.11663
Published
1988-12-23
Pages
11663-73
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339102
Subset
IM
Grants
NIGMS NIH HHS · GM-33448 · United States
External Links
PubMed source DOI: 10.1093/nar/16.24.11663 PMC Full Text View journal details
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