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PMID: 2836799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cDNA cloning of human hnRNP protein A1 reveals the existence of multiple mRNA isoforms.

Nucleic acids research ·Vol. 16 ·No. 9 ·1988-05-11 ·Pages 3751-70

Buvoli M, Biamonti G, Tsoulfas P, Bassi MT, Ghetti A, Riva S, Morandi C

Abstract

Protein A1 is one of the major component of mammalian ribonucleoprotein particles (hnRNP). Human protein A1 cDNA cloning and sequencing revealed the existence of at least two protein isoforms. Among the cDNAs examined, sequence differences were found both in the structural portion, leading to aminoacid changes (Tyr to Phe or Arg to Lys) and in the non translated 3'-region where two T-stretches of different length were observed. Interestingly one of the aminoacid substitutions falls into a consensus sequence common to many RNA binding proteins. Northern blot analysis of poly A+ RNAs from five human tissues revealed two mRNA forms of 1500 and 1900 n due to alternative polyadenylation. Analysis of genomic DNA showed at least 30 A1-specific sequences, some of which correspond to processed pseudogenes. These results suggest that protein A1 is encoded by a multigene family.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics DNA Restriction Enzymes Genes Genetic Variation Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Humans Molecular Sequence Data Nucleotide Mapping Organ Specificity RNA, Messenger/genetics Ribonucleoproteins/genetics Transcription, Genetic
Chemicals
Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins RNA, Messenger Ribonucleoproteins DNA DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Buvoli M
Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Pavia, Italy.
Biamonti G
Tsoulfas P
Bassi M T
Ghetti A
Riva S
Morandi C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.9.3751
Published
1988-05-11
Pages
3751-70
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336554
Subset
IM
Databases
GENBANK
X06747
External Links
PubMed source DOI: 10.1093/nar/16.9.3751 PMC Full Text View journal details
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