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PMID: 12853574 Published · ppublish English Journal Article

Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 15 ·2003-07-22 ·Pages 8823-7

Tariq M, Saze H, Probst AV, Lichota J, Habu Y, Paszkowski J

Abstract

In mammals and plants, formation of heterochromatin is associated with hypermethylation of DNA at CpG sites and histone H3 methylation at lysine 9. Previous studies have revealed that maintenance of DNA methylation in Neurospora and Arabidopsis requires histone H3 methylation. A feedback loop from DNA methylation to histone methylation, however, is less understood. Its recent examination in Arabidopsis with a partial loss of function in DNA methyltransferase 1 (responsible for maintenance of CpG methylation) yielded conflicting results. Here we report that complete removal of CpG methylation in an Arabidopsis mutant null for DNA maintenance methyltransferase results in a clear loss of histone H3 methylation at lysine 9 in heterochromatin and also at heterochromatic loci that remain transcriptionally silent. Surprisingly, these dramatic alterations are not reflected in heterochromatin relaxation.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins CpG Islands DNA (Cytosine-5-)-Methyltransferases/genetics,metabolism DNA Methylation DNA, Plant/chemistry,genetics Heterochromatin/chemistry,genetics Histones/chemistry,genetics Lysine/chemistry
Chemicals
Arabidopsis Proteins DNA, Plant Heterochromatin Histones MET1 protein, Arabidopsis DNA (Cytosine-5-)-Methyltransferases Lysine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tariq Muhammad
Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland. [email protected]
Saze Hidetoshi
Probst Aline V
Lichota Jacek
Habu Yoshiki
Paszkowski Jerzy
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1432939100
Email
[email protected]
Published
2003-07-22
Epub
2003-00-09
Pages
8823-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC166397
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.1432939100 PMC Full Text View journal details
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