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PMID: 12856951 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Active gene expression of a xyloglucan endotransglucosylase/hydrolase gene, XTH9, in inflorescence apices is related to cell elongation in Arabidopsis thaliana.

Plant molecular biology ·Vol. 52 ·No. 2 ·2003-05-00 ·Pages 473-82

Hyodo H, Yamakawa S, Takeda Y, Tsuduki M, Yokota A, Nishitani K, Kohchi T

Abstract

Regulation of cell wall structure plays a central role in growth and differentiation in plants. Xyloglucan endotransglucosylase/hydrolases (XTHs) that catalyze the cleavage and molecular grafting of xyloglucan chains function in loosening and rearrangement of the cell wall. We have characterized XTH9, a member of the XTH family that was isolated by systematic differential screening for highly expressed genes in shoot apices in Arabidopsis. In the vegetative phase, XTH9 transcripts accumulate in the shoot apex region. In the reproductive phase, transcript levels in shoot apices increase further, and they also are detected in flower buds, flower stalks and internodes bearing flowers. XTH9 expression levels were reduced markedly in mutants such as acl which are characterized by short internodal cell lengths, but recover at permissive temperatures in the temperature-sensitive acl mutants. Differential expression of XTH9 along the inflorescence stem was also detected in pin1 where no lateral organs are formed. These observations suggest that XTH9 expression is coordinated with plant development, including the differentiation from vegetative and reproductive meristems and cell elongation of the inflorescence stem.

MeSH Terms
Amino Acid Sequence Arabidopsis/cytology,enzymology,genetics Arabidopsis Proteins/genetics Base Sequence Cell Division/genetics DNA, Complementary/chemistry,genetics Flowers/enzymology,genetics,growth & development Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Glycosyltransferases/genetics In Situ Hybridization Molecular Sequence Data Mutation RNA, Plant/genetics,metabolism Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
Arabidopsis Proteins DNA, Complementary RNA, Plant Glycosyltransferases xyloglucan - xyloglucosyltransferase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hyodo Hideki
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Yamakawa Seiyei
Takeda Yuji
Tsuduki Masao
Yokota Akiho
Nishitani Kazuhiko
Kohchi Takayuki
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1023904217641
Published
2003-05-00
Pages
473-82
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1023904217641 View journal details
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