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PMID: 12909721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ELG1, a yeast gene required for genome stability, forms a complex related to replication factor C.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 17 ·2003-08-19 ·Pages 9906-11

Ben-Aroya S, Koren A, Liefshitz B, Steinlauf R, Kupiec M

Abstract

Many overlapping surveillance and repair mechanisms operate in eukaryotic cells to ensure the stability of the genome. We have screened to isolate yeast mutants exhibiting increased levels of recombination between repeated sequences. Here we characterize one of these mutants, elg1. Strains lacking Elg1p exhibit elevated levels of recombination between homologous and nonhomologous chromosomes, as well as between sister chromatids and direct repeats. These strains also exhibit increased levels of chromosome loss. The Elg1 protein shares sequence homology with the large subunit of the clamp loader replication factor C (RFC) and with the product of two additional genes involved in checkpoint functions and genome maintenance: RAD24 and CTF18. Elg1p forms a complex with the Rfc2-5 subunits of RFC that is distinct from the previously described RFC-like complexes containing Rad24 and Ctf18. Genetic data indicate that the Elg1, Ctf18, and Rad24 RFC-like complexes work in three separate pathways important for maintaining the integrity of the genome and for coping with various genomic stresses.

MeSH Terms
Amino Acid Sequence Carrier Proteins/genetics Crossing Over, Genetic DNA Replication/genetics DNA-Binding Proteins/genetics Gene Conversion Gene Deletion Genes, Fungal Genome, Fungal Molecular Sequence Data Mutation Proteins Recombination, Genetic Replication Protein C Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics Sequence Homology, Amino Acid Sister Chromatid Exchange
Chemicals
CTF4 protein, S cerevisiae Carrier Proteins DNA-Binding Proteins Elg1 protein, S cerevisiae Proteins Saccharomyces cerevisiae Proteins Replication Protein C
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ben-Aroya Shay
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel.
Koren Amnon
Liefshitz Batia
Steinlauf Rivka
Kupiec Martin
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1633757100
Published
2003-08-19
Epub
2003-00-08
Pages
9906-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC187881
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.1633757100 PMC Full Text View journal details
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