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PMID: 7651383 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of the five replication factor C genes of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 15 ·No. 9 ·1995-09-00 ·Pages 4661-71

Cullmann G, Fien K, Kobayashi R, Stillman B

Abstract

Replication factor C (RFC) is a five-subunit DNA polymerase accessory protein that functions as a structure-specific, DNA-dependent ATPase. The ATPase function of RFC is activated by proliferating cell nuclear antigen. RFC was originally purified from human cells on the basis of its requirement for simian virus 40 DNA replication in vitro. A functionally homologous protein complex from Saccharomyces cerevisiae, called ScRFC, has been identified. Here we report the cloning, by either peptide sequencing or by sequence similarity to the human cDNAs, of the S. cerevisiae genes RFC1, RFC2, RFC3, RFC4, and RFC5. The amino acid sequences are highly similar to the sequences of the homologous human RFC 140-, 37-, 36-, 40-, and 38-kDa subunits, respectively, and also show amino acid sequence similarity to functionally homologous proteins from Escherichia coli and the phage T4 replication apparatus. All five subunits show conserved regions characteristic of ATP/GTP-binding proteins and also have a significant degree of similarity among each other. We have identified eight segments of conserved amino acid sequences that define a family of related proteins. Despite their high degree of sequence similarity, all five RFC genes are essential for cell proliferation in S. cerevisiae. RFC1 is identical to CDC44, a gene identified as a cell division cycle gene encoding a protein involved in DNA metabolism. CDC44/RFC1 is known to interact genetically with the gene encoding proliferating cell nuclear antigen, confirming previous biochemical evidence of their functional interaction in DNA replication.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution Cell Cycle Proteins/genetics Cell Division/genetics Cloning, Molecular DNA-Binding Proteins/genetics DNA-Directed DNA Polymerase/genetics Genes, Fungal/genetics Genes, Lethal/genetics Homeodomain Proteins Humans Infant, Newborn Minor Histocompatibility Antigens Molecular Sequence Data Mutagenesis Proto-Oncogene Proteins c-bcl-2 Replication Protein C Repressor Proteins Restriction Mapping Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Species Specificity
Chemicals
BCL2-related protein A1 Cell Cycle Proteins DNA-Binding Proteins Homeodomain Proteins MATA1 protein, S cerevisiae Minor Histocompatibility Antigens Proto-Oncogene Proteins c-bcl-2 RFC1 protein, S cerevisiae Repressor Proteins Saccharomyces cerevisiae Proteins DNA-Directed DNA Polymerase Replication Protein C
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cullmann G
Cold Spring Harbor Laboratory, New York 11724, USA.
Fien K
Kobayashi R
Stillman B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.9.4661
Published
1995-09-00
Pages
4661-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230709
Subset
IM
Grants
NIAID NIH HHS · AI20460 · United States
NCI NIH HHS · CA13106 · United States
Databases
GENBANK
U26027, U26028, U26029, U26030, U26031
External Links
PubMed source DOI: 10.1128/MCB.15.9.4661 PMC Full Text View journal details
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