Home LiteratureArticle Details
PMID: 8302859 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning and expression of the Saccharomyces cerevisiae RFC3 gene, an essential component of replication factor C.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 3 ·1994-02-01 ·Pages 868-72

Li X, Burgers PM

Abstract

Yeast replication factor C (RF-C) is a multi-polypeptide complex required for processive DNA replication by DNA polymerases delta and epsilon. The gene encoding the 40-kDa subunit of the Saccharomyces cerevisiae RF-C (RFC3) has been cloned. The RFC3 gene is required for yeast cell growth and has been mapped to the left arm of chromosome XIV. The deduced amino acid sequence of the RFC3 gene shows a high homology to the 36-, 37-, and 40-kDa subunits of human RF-C (also called activator 1), with the highest homology to the 36-kDa subunit. Among the conserved regions are the A motif of ATP binding proteins; the "DEAD box," common to DNA helicases and other ATPases; and the "RFC box," an approximately 15-amino acid domain virtually identical in the yeast and human RF-C subunits. Limited homology to the functional homologs of the Escherichia coli replication apparatus was also observed. The steady-state mRNA levels of RFC3 do not change significantly during the mitotic cell cycle of yeast. The intact form of the RFC3 gene product (Rfc3p) has been overproduced in E. coli and purified to homogeneity. Purified Rfc3p has an ATPase activity that is markedly stimulated by single-stranded DNA but not by double-stranded DNA or RNA.

Related Genes
RFC3
MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Amino Acid Sequence Chromosome Mapping Cloning, Molecular DNA Replication/genetics DNA-Binding Proteins/chemistry,genetics,metabolism Escherichia coli/genetics Gene Expression Genes, Fungal Homeodomain Proteins Humans Minor Histocompatibility Antigens Molecular Sequence Data Proto-Oncogene Proteins c-bcl-2 Replication Protein C Repressor Proteins Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Species Specificity
Chemicals
BCL2-related protein A1 DNA-Binding Proteins Homeodomain Proteins MATA1 protein, S cerevisiae Minor Histocompatibility Antigens Proto-Oncogene Proteins c-bcl-2 Repressor Proteins Saccharomyces cerevisiae Proteins Adenosine Triphosphatases Replication Protein C
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Li X
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110.
Burgers P M
References (34)
34 references, click to expand
  1. Synthesis of DNA by DNA polymerase epsilon in vitro.
    J Biol Chem. 1991 Nov 25;266(33):22707-17 PMID: 1682323
  2. Saccharomyces cerevisiae replication factor C. II. Formation and activity of complexes with the proliferating cell nuclear antigen and with DNA polymerases delta and epsilon.
    J Biol Chem. 1991 Nov 25;266(33):22698-706 PMID: 1682322
  3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  4. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  5. DNA polymerase III from Saccharomyces cerevisiae. I. Purification and characterization.
    J Biol Chem. 1988 Jan 15;263(2):917-24 PMID: 3275661
  6. DNA polymerase III, a second essential DNA polymerase, is encoded by the S. cerevisiae CDC2 gene.
    Cell. 1989 Feb 24;56(4):599-605 PMID: 2645055
  7. Identification of replication factor C from Saccharomyces cerevisiae: a component of the leading-strand DNA replication complex.
    Mol Cell Biol. 1992 Jan;12(1):155-63 PMID: 1346062
  8. Sequence and expression in Escherichia coli of the 40-kDa subunit of activator 1 (replication factor C) of HeLa cells.
    Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2516-20 PMID: 1313560
  9. Studies of the cloned 37-kDa subunit of activator 1 (replication factor C) of HeLa cells.
    Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5211-5 PMID: 1351677
  10. Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6.
    Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9479-83 PMID: 1409658
  11. A Saccharomyces cerevisiae DNA helicase associated with replication factor C.
    J Biol Chem. 1992 Dec 15;267(35):25321-7 PMID: 1460028
  12. The subunits of activator 1 (replication factor C) carry out multiple functions essential for proliferating-cell nuclear antigen-dependent DNA synthesis.
    Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):6-10 PMID: 8093561
  13. Homology in accessory proteins of replicative polymerases--E. coli to humans.
    Nucleic Acids Res. 1993 Jan 11;21(1):1-3 PMID: 8441605
  14. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs.
    Genetics. 1993 May;134(1):81-150 PMID: 8514151
  15. DNA polymerase alpha.
    J Biol Chem. 1989 Mar 15;264(8):4265-8 PMID: 2647732
  16. Purification of a cellular replication factor, RF-C, that is required for coordinated synthesis of leading and lagging strands during simian virus 40 DNA replication in vitro.
    Mol Cell Biol. 1989 Feb;9(2):609-19 PMID: 2565531
  17. Structure and function of the Saccharomyces cerevisiae CDC2 gene encoding the large subunit of DNA polymerase III.
    EMBO J. 1989 Jun;8(6):1849-54 PMID: 2670563
  18. Eukaryotic DNA polymerases alpha and delta: conserved properties and interactions, from yeast to mammalian cells.
    Prog Nucleic Acid Res Mol Biol. 1989;37:235-80 PMID: 2505329
  19. Functions of replication factor C and proliferating-cell nuclear antigen: functional similarity of DNA polymerase accessory proteins from human cells and bacteriophage T4.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):1023-7 PMID: 1967833
  20. Molecular cloning, structure and expression of the yeast proliferating cell nuclear antigen gene.
    Nucleic Acids Res. 1990 Jan 25;18(2):261-5 PMID: 1970160
  21. Mechanism of elongation of primed DNA by DNA polymerase delta, proliferating cell nuclear antigen, and activator 1.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):5672-6 PMID: 1974050
  22. Revised nomenclature for eukaryotic DNA polymerases.
    Eur J Biochem. 1990 Aug 17;191(3):617-8 PMID: 2390988
  23. A third essential DNA polymerase in S. cerevisiae.
    Cell. 1990 Sep 21;62(6):1143-51 PMID: 2169349
  24. Studies on the activator 1 protein complex, an accessory factor for proliferating cell nuclear antigen-dependent DNA polymerase delta.
    J Biol Chem. 1991 Jan 5;266(1):594-602 PMID: 1670772
  25. Replication factors required for SV40 DNA replication in vitro. I. DNA structure-specific recognition of a primer-template junction by eukaryotic DNA polymerases and their accessory proteins.
    J Biol Chem. 1991 Jan 25;266(3):1950-60 PMID: 1671045
  26. Replication factors required for SV40 DNA replication in vitro. II. Switching of DNA polymerase alpha and delta during initiation of leading and lagging strand synthesis.
    J Biol Chem. 1991 Jan 25;266(3):1961-8 PMID: 1671046
  27. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
    Methods Enzymol. 1991;194:281-301 PMID: 2005793
  28. Construction of deletion mutants of the Escherichia coli UvrA protein and their purification from inclusion bodies.
    J Biol Chem. 1991 Jun 15;266(17):11380-7 PMID: 1645734
  29. The 3' to 5' exonuclease activity located in the DNA polymerase delta subunit of Saccharomyces cerevisiae is required for accurate replication.
    EMBO J. 1991 Aug;10(8):2165-70 PMID: 1648480
  30. Eukaryotic DNA polymerases.
    Annu Rev Biochem. 1991;60:513-52 PMID: 1883203
  31. Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcription.
    Cell. 1991 Sep 6;66(5):1015-26 PMID: 1832336
  32. The spectrum of spontaneous mutations in a Saccharomyces cerevisiae uracil-DNA-glycosylase mutant limits the function of this enzyme to cytosine deamination repair.
    J Bacteriol. 1991 Nov;173(21):6807-10 PMID: 1938887
  33. Saccharomyces cerevisiae replication factor C. I. Purification and characterization of its ATPase activity.
    J Biol Chem. 1991 Nov 25;266(33):22689-97 PMID: 1682321
  34. Primary structure of the catalytic subunit of calf thymus DNA polymerase delta: sequence similarities with other DNA polymerases.
    Biochemistry. 1991 Dec 24;30(51):11742-50 PMID: 1721537
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.3.868
Published
1994-02-01
Pages
868-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC521413
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032431 · United States
NIGMS NIH HHS · GM32431 · United States
Databases
GENBANK
L18755
External Links
PubMed source DOI: 10.1073/pnas.91.3.868 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2