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PMID: 12913166 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity.

Plant physiology ·Vol. 132 ·No. 4 ·2003-08-00 ·Pages 2116-25

Danna CH, Bartoli CG, Sacco F, Ingala LR, Santa-María GE, Guiamet JJ, Ugalde RA

Abstract

In chloroplasts, stromal and thylakoid-bound ascorbate peroxidases (tAPX) play a major role in the removal of H(2)O(2) produced during photosynthesis. Here, we report that hexaploid wheat (Triticum aestivum) expresses three homeologous tAPX genes (TaAPX-6A, TaAPX-6B, and TaAPX-6D) mapping on group-6 chromosomes. The tAPX activity of a mutant line lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.

MeSH Terms
Amino Acid Sequence Ascorbate Peroxidases Ascorbic Acid/metabolism Base Sequence Electron Transport Gene Expression Profiling Genes, Plant/genetics Genome, Plant Glutathione/metabolism Molecular Sequence Data Mutation/genetics Oxidative Stress Peroxidases/genetics,metabolism Photons Photosynthesis Plant Leaves/enzymology,genetics,growth & development,metabolism Ploidies Sequence Alignment Thylakoids/enzymology,genetics Triticum/enzymology,genetics,growth & development,metabolism
Chemicals
Peroxidases Ascorbate Peroxidases Glutathione Ascorbic Acid
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Danna Cristian H
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Avenida General Paz entre Albarellos y Constituyentes, Instituto Nacional de Tecnología Industrial, CP 1650, San Martín, Provincia de Buenos Aires, Argentina.
Bartoli Carlos G
Sacco Francisco
Ingala Lorena R
Santa-María Guillermo E
Guiamet Juan J
Ugalde Rodolfo A
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.103.021717
Published
2003-08-00
Pages
2116-25
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC181295
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.103.021717 PMC Full Text View journal details
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