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PMID: 12923297 Published · ppublish English Comparative Study Journal Article

Genetic and functional differences between multipotent neural and pluripotent embryonic stem cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 Suppl 1 ·2003-09-30 ·Pages 11866-72

D'Amour KA, Gage FH

Abstract

Stem cells (SCs) are functionally defined by their abilities to self-renew and generate differentiated cells. Although much effort has been focused on defining the common characteristics among various types of SCs, the genetic and functional differences between multipotent and pluripotent SCs have garnered less attention. We report a direct genetic and functional comparison of molecularly defined and clonally related populations of neural SCs (NSCs) and embryonic SCs (ESCs), using the Sox2 promoter for isolation of purified populations by fluorescence-activated cell sorting. A stringent expression profile comparison of promoter-defined NSCs and ESCs revealed a striking dissimilarity, and subsequent chimera analyses confirmed the fundamental differences in cellular potency between these populations. This direct comparison elucidates the molecular basis for the functional differences in pluripotent ESCs and multipotent NSCs.

MeSH Terms
Animals Base Sequence DNA, Complementary/genetics DNA-Binding Proteins/genetics Gene Expression Profiling Green Fluorescent Proteins HMGB Proteins Luminescent Proteins/genetics Mice Mice, Transgenic Multipotent Stem Cells/cytology,physiology Neurons/chemistry,physiology Nuclear Proteins/genetics Pluripotent Stem Cells/cytology,physiology Promoter Regions, Genetic Recombinant Fusion Proteins/genetics Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors Transcription Factors
Chemicals
DNA, Complementary DNA-Binding Proteins HMGB Proteins Luminescent Proteins Nuclear Proteins Recombinant Fusion Proteins SOXB1 Transcription Factors Sox2 protein, mouse Transcription Factors Green Fluorescent Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
D'Amour Kevin A
Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Gage Fred H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1834200100
Published
2003-09-30
Epub
2003-00-15
Pages
11866-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304100
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.1834200100 PMC Full Text View journal details
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