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PMID: 12928499 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 18 ·2003-09-02 ·Pages 10181-6

Buell CR, Joardar V, Lindeberg M, Selengut J, Paulsen IT, Gwinn ML, Dodson RJ, Deboy RT, Durkin AS, Kolonay JF, Madupu R, Daugherty S, Brinkac L, Beanan MJ, Haft DH, Nelson WC, Davidsen T, Zafar N, Zhou L, Liu J, Yuan Q, Khouri H, Fedorova N, Tran B, Russell D, Berry K, Utterback T, Van Aken SE, Feldblyum TV, D'Ascenzo M, Deng WL, Ramos AR, Alfano JR, Cartinhour S, Chatterjee AK, Delaney TP, Lazarowitz SG, Martin GB, Schneider DJ, Tang X, Bender CL, White O, Fraser CM, Collmer A

Abstract

We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise 7% of the DC3000 genome. The bacterium possesses a large repertoire of transporters for the acquisition of nutrients, particularly sugars, as well as genes implicated in attachment to plant surfaces. Over 12% of the genes are dedicated to regulation, which may reflect the need for rapid adaptation to the diverse environments encountered during epiphytic growth and pathogenesis. Comparative analyses confirmed a high degree of similarity with two sequenced pseudomonads, Pseudomonas putida and Pseudomonas aeruginosa, yet revealed 1,159 genes unique to DC3000, of which 811 lack a known function.

MeSH Terms
Arabidopsis/microbiology Base Sequence Biological Transport Genome, Bacterial Lycopersicon esculentum/microbiology Molecular Sequence Data Plant Growth Regulators/biosynthesis Plasmids Pseudomonas/genetics,metabolism,pathogenicity Reactive Oxygen Species Siderophores/biosynthesis Virulence
Chemicals
Plant Growth Regulators Reactive Oxygen Species Siderophores
Authors & Affiliations
44 authors, click to expand affiliations / ORCID
Buell C Robin
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. [email protected]
Joardar Vinita
Lindeberg Magdalen
Selengut Jeremy
Paulsen Ian T
Gwinn Michelle L
Dodson Robert J
Deboy Robert T
Durkin A Scott
Kolonay James F
Madupu Ramana
Daugherty Sean
Brinkac Lauren
Beanan Maureen J
Haft Daniel H
Nelson William C
Davidsen Tanja
Zafar Nikhat
Zhou Liwei
Liu Jia
Yuan Qiaoping
Khouri Hoda
Fedorova Nadia
Tran Bao
Russell Daniel
Berry Kristi
Utterback Teresa
Van Aken Susan E
Feldblyum Tamara V
D'Ascenzo Mark
Deng Wen-Ling
Ramos Adela R
Alfano James R
Cartinhour Samuel
Chatterjee Arun K
Delaney Terrence P
Lazarowitz Sondra G
Martin Gregory B
Schneider David J
Tang Xiaoyan
Bender Carol L
White Owen
Fraser Claire M
Collmer Alan
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1731982100
Email
[email protected]
Published
2003-09-02
Epub
2003-00-19
Pages
10181-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC193536
Subset
IM
Grants
NIAID NIH HHS · AI 43311 · United States
Databases
GENBANK
AE016853, AE016854, AE016855
External Links
PubMed source DOI: 10.1073/pnas.1731982100 PMC Full Text View journal details
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